The potential of spectral reflectance technique for the detection of Grapevine leafroll-associated virus-3 in two red-berried wine grape cultivars
The potential of spectral reflectance technique for the detection of Grapevine leafroll-associated virus-3 in two red-berried wine grape cultivars
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DOI：
10.1016/j.compag.2008.11.007
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发表时间：
2009-04-01
影响因子：
8.3
通讯作者：
Mekuria, Tefera
中科院分区：
文献类型：
--
作者：
Naidu, Rayapati A.;Perry, Eileen M.;Mekuria, Tefera
检测植物中的病毒涉及破坏性取样，然后通过酶联免疫吸附测定（ELISA）和/或逆转录聚合酶链反应（RT-PCR）进行检测。在这项研究中，我们研究了病毒感染和未感染葡萄（葡萄属vinifera L）之间叶片光谱反射率变化的潜力，以开发用于葡萄藤叶病现场“实时”诊断的非侵入性技术（GLD）。用便携式光谱仪对两个酿酒葡萄品种（赤霞珠和梅洛）的未感染和葡萄藤相关病毒3（GLRaV-3）感染植株的离体叶片进行原位叶片反射光谱。在绿色峰（近550 nm）、近红外（近900 nm）和中红外（近1600 nm和2200 nm）中，病毒感染和未感染叶片的植被指数和波长间隔存在特定差异。反射光谱和分类分析的结果表明，不同的植被指数和/或个人的波长带可能会有所不同，在他们的能力，以检测GLD取决于是否有明显的症状，在病毒感染的叶子。在特定波长间隔的叶片反射率测量的差异之间的病毒感染和未感染的葡萄和它们的相关性与RT-PCR结果的存在GLRaV-3建议光谱反射技术作为一个有前途的工具，成本效益高，无损的方法诊断GLD在现场。据我们所知，这是第一个研究报告的潜力，利用叶光谱数据的病毒病诊断在多年生作物。(C)2008 Elsevier B. V.保留所有权利。
The detection of viruses in plants involves destructive sampling followed by testing by enzyme-linked immunosorbent assay (ELISA) and/or reverse transcription-polymerase chain reaction (RT-PCR). In this study, we have investigated the potential of leaf spectral reflectance changes between virus infected and uninfected grapevines (Vitis vinifera L) in developing non-invasive techniques for field-based 'real-time' diagnosis of grapevine leafroll disease (GLD). In situ leaf reflectance spectra were taken with a portable spectrometer using detached leaves from uninfected and Grapevine leafroll-associated virus-3 (GLRaV-3) infected plants of two wine grape cultivars (Cabernet Sauvignon and Merlot). Specific differences in vegetation indices and wavelength intervals were observed between virus-infected and uninfected leaves in the green peak (near 550 nm), the near infrared (near 900 nm) and in the mid-infrared (near 1600 nm and 2200 nm). Results of reflectance spectra and classification analysis suggest that different vegetation indices and/or individual wavelength bands may differ in their ability to detect GLD depending on whether there are visible symptoms in the virus-infected leaves. The differences in leaf reflectance measurements at specific wavelength intervals between virus-infected and uninfected grapevines and their correlation with RT-PCR results for the presence of GLRaV-3 suggest spectral reflectance technique as a promising tool for cost-effective, nondestructive method for diagnosis of GLD in the field. To our knowledge this represents the first study to report the potential of using leaf spectral data for virus disease diagnosis in a perennial crop. (C) 2008 Elsevier B.V. All rights reserved.