Point-of-care fluorescence immunoassay for prostate specific antigen
Point-of-care fluorescence immunoassay for prostate specific antigen
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DOI：
10.1016/j.cca.2009.04.013
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发表时间：
2009-08-11
影响因子：
5
通讯作者：
Choi, Eui Yul
中科院分区：
文献类型：
--
作者：
Oh, Sang Wook;Kim, Young Min;Choi, Eui Yul
背景：前列腺特异性抗原（PSA）作为前列腺癌的诊断、筛查和预后的临床标志物被广泛应用。方法：将全血或血清与荧光染料结合的检测剂混合后，加入荧光检测柱中，孵育12 min，用激光荧光扫描仪测定荧光强度。通过精密度和回收率试验评价了FL-ICA系统的分析性能。结果：反映供试品/对照品荧光强度的面积比（A（T）/A（C））与PSA浓度呈良好的相关性（r = 0.998）。在2.5-8 μ g/l范围内，试验内和试验间精密度的CV为
Background: Prostate specific antigen (PSA) is widely used as a clinical marker for diagnosis, screening, and prognosis of prostate cancer. A fluorescence (FL) dye-incorporated immunochromatographic assay (ICA) was developed for detection of PSA concentration in whole blood or serum.Methods: Whole blood or serum mixed with FL-conjugated detector was loaded onto a cartridge and incubated for 12 min. The FL intensity of the cartridge was then measured in a laser FL scanner. The analytical performance of the FL-ICA system was evaluated by precision and recovery tests. The comparability of the new method was examined with an automated analyzer.Results: A reliable correlation between area ratio (A(T)/A(C)), reflecting FL intensity of test/control line, and PSA concentration was observed (r = 0.998). The CVs of intra- and inter-assay precision in a range of 2.5-8 mu g/l were