The relative expression amounts of apterous and its co-factor dLdb/Chip are critical for dorso-ventral compartmentalization in the Drosophila wing
The relative expression amounts of apterous and its co-factor dLdb/Chip are critical for dorso-ventral compartmentalization in the Drosophila wing
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DOI：
10.1093/emboj/17.23.6846
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发表时间：
1998-12-01
期刊：
影响因子：
11.4
通讯作者：
Botas, J
中科院分区：
文献类型：
--
作者：
Fernández-Fúnez, P;Lu, CH;Botas, J
果蝇翅膀中背腹轴的形成需要背细胞中 Apterous LIM/同源域蛋白 (Ap) 的局部积累。在这里，我们报告 dLdb/Chip 编码控制 Ap 活性的 LIM 结合辅因子。 dLdb/Chip 功能的缺乏和过度都会导致与 apterous tap 相同的表型）功能缺乏；即背侧到腹侧的转变、新翼缘的产生和翼的生长。这些结果表明，Ap 在背腹区室化中的正常功能需要正确量的 DLDB/CHIP 辅因子，并表明 Ap 和 DLDB/CHIP 蛋白形成多聚体功能复合物。为了支持该模型，我们表明 dLdb/Chip 功能过剩表型可以通过 ap 过表达来挽救。
Dorso-ventral axis formation in the Drosophila wing requires the localized accumulation of the Apterous LIM/homeodomain protein (Ap) in dorsal cells. Here we report that dLdb/Chip encodes a LIM-binding cofactor that controls Ap activity. Both lack and excess of dLdb/Chip function cause the same phenotype as apterous tap) lack of function; i.e. dorsal to ventral transformations, generation of new wing margins, and wing outgrowths. These results indicate that the normal function of Ap in dorso-ventral compartmentalization requires the correct amount of the DLDB/CHIP cofactor, and suggest that the Ap and DLDB/CHIP proteins form a multimeric functional complex, In support of this model, we show that the dLdb/Chip excess-of-function phenotypes can be rescued by ap overexpression.