Characterization and analysis of frog photoreceptor membranes.

Characterization and analysis of frog photoreceptor membranes.
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DOI:
10.1085/jgp.58.3.225
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发表时间:
1971-09
期刊:
The Journal of general physiology
影响因子:
--
通讯作者:
Robinson W
Robinson W
中科院分区:
其他
文献类型:
--
作者:
Bownds D;Gordon-Walker A;Gaide-Huguenin AC;Robinson W

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青蛙光感受器膜每摩尔视觉色素发色团含有54,000克蛋白质,几乎所有的都是不溶于膜的蛋白质。丙烯酰胺凝胶电泳显示一个主要的多肽类别,最有可能的是视觉色素载脂蛋白。当有三磷酸腺苷存在时,这些光感受器膜的悬浮液会积累钙离子,这一特征可能在视觉兴奋中发挥作用。
Frog photoreceptor membranes contain 54,000 g of protein per mole of visual pigment chromophore, virtually all of it insoluble membrane protein. Acrylamide gel electrophoresis indicates one major polypeptide class, most likely the visual pigment apoprotein. Suspensions of these photoreceptor membranes accumulate calcium ions when ATP is present, a characteristic that may play a part in visual excitation.