Establishment and characterization of an amylase-producing human myeloma cell line.
Establishment and characterization of an amylase-producing human myeloma cell line.
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产生淀粉酶的人骨髓瘤细胞系的建立和表征。
DOI：
10.1182/blood.v72.3.978.978
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发表时间：
1988
期刊：
影响因子：
20.3
通讯作者：
K. Takatsuki
中科院分区：
文献类型：
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作者：
H. Matsuzaki;H. Hata;M. Takeya;K. Takatsuki
从合并高淀粉酶血症的多发性骨髓瘤患者身上建立了两个稳定的产生IgA lambda的浆细胞系（KHM-1A和KHM-1B），它们不含Epstein-Barr病毒。两种细胞系的表面标记研究表明，细胞表面不含免疫球蛋白，但细胞质免疫球蛋白（IgA lambda）、HLA-DR和PCA-1阳性。通过斑块形成细胞试验和培养基酶联免疫吸附试验检测这两种细胞系分泌的IgA单克隆免疫球蛋白。KHM-1B细胞也分泌α -淀粉酶，但在培养条件下的KHM-1A上清液中未检测到这种活性。
Two stable lines of IgA lambda-producing plasma cells (KHM-1A and KHM-1B) that were free of the Epstein-Barr virus were established from a patient with multiple myeloma complicated by hyperamylasemia. Surface marker studies of the two cell lines showed that the cells had no surface immunoglobulins but were positive for cytoplasmic immunoglobulins (IgA lambda) and for HLA-DR and PCA-1. Secretion of IgA monoclonal immunoglobulin by the two lines was detected by a plaque-forming cell assay and by an enzyme-linked immunosorbent assay of culture media. KHM-1B cells also secreted alpha-amylase, but no such activity was detected in the culture-conditioned supernatant fluid of KHM-1A.
通过单克隆抗体鉴定人浆细胞上的抗原。
DOI：
--
发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Anderson,KC;Park,EK;Bates,MP;Leonard,RC;Hardy,R;Schlossman,SF;Nadler,LM
通讯作者：
Nadler,LM
从双侧胸腔积液中建立两种新的骨髓瘤细胞系：体内连续克隆变化的证据。
影响因子：
20.3
作者：
Durie,BG;Vela,E;Baum,V;Leibovitz,A;Payne,CM;Richter,LC;Grogan,TM;Trent,JM
通讯作者：
Trent,JM