Biophysical Analyses for Probing Glycan-Protein Interactions
Biophysical Analyses for Probing Glycan-Protein Interactions
复制标题
用于探测聚糖-蛋白质相互作用的生物物理分析
DOI：
10.1007/978-981-13-2158-0_7
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Yamaguchi Yoshiki
中科院分区：
文献类型：
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作者：
Nagae Masamichi;Yamaguchi Yoshiki
聚糖-蛋白相互作用发生在许多生理事件中，分析对于理解聚糖依赖机制具有相当重要的意义。包括三维结构分析在内的生物物理学方法对于揭示聚糖-蛋白质在原子水平上的相互作用至关重要。聚糖固有的多样性使它们适合作为识别标签的功能，例如，区分病原体的自我和非自我成分。然而，聚糖的复杂性和对相互作用伙伴的亲和力差限制了传统分析的有用性。为了处理这些麻烦的聚糖，需要发展出生物物理分析的逻辑序列。在本章中，我们介绍了一个由六个步骤组成的聚糖-蛋白相互作用分析的工作流程：凝集素和聚糖的制备、聚糖配体的筛选、结合表位的确定、定量相互作用分析、三维结构分析和分子动力学模拟。我们对凝集素-聚糖相互作用的知识和理解的增加将有望导致基于糖的药物和疫苗的设计。
Glycan-protein interactions occur at many physiological events, and the analyses are of considerable importance for understanding glycan-dependent mechanisms. Biophysical approaches including 3D structural analysis are essential for revealing glycan-protein interactions at the atomic level. The inherent diversity of glycans suits them to function as identification tags, e.g., distinguish self from the nonself components of pathogens. However, the complexity of glycans and poor affinities for interaction partners limit the usefulness of conventional analyses. To cope with such troublesome glycans, a logical sequence of biophysical analyses need to be developed. In this chapter, we introduce a workflow of glycan-protein interaction analysis consisting of six steps: preparation of lectin and glycan, screening of glycan ligand, determination of binding epitope, quantitative interaction analysis, 3D structural analysis, and molecular dynamics simulation. Our increasing knowledge and understanding of lectin-glycan interactions will hopefully lead to the design of glyco-based medicines and vaccines.