COLLAGEN FIBRILLOGENESIS INSITU - FIBRIL SEGMENTS ARE INTERMEDIATES IN MATRIX ASSEMBLY
COLLAGEN FIBRILLOGENESIS INSITU - FIBRIL SEGMENTS ARE INTERMEDIATES IN MATRIX ASSEMBLY
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DOI：
10.1073/pnas.86.12.4549
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发表时间：
1989-06-01
影响因子：
11.1
通讯作者：
TRELSTAD, RL
中科院分区：
文献类型：
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作者：
BIRK, DE;ZYCBAND, EI;TRELSTAD, RL
用连续切片、透射电子显微镜和计算机图像重建技术对14日龄鸡胚腱的不连续纤维段和束的组装进行了研究。原纤段最早发现于胞质外通道，也就是它们聚合的部位；它们也在原纤束内发现。在连续的部分中，在其整个长度上跟随单个原纤维片段，并且它们的长度范围为7到15微米。确定了纤维片段末端的结构差异，表明纤维片段的氨基/羧基极性反映在其结构中。我们的数据表明，纤维段是胶原纤维形成的前体，我们认为纤维段的沉积后融合可能是肌腱发育和生长的一个重要过程。
The assembly of discontinuous fibril segments and bundles was studied in 14-day chicken embryo tendons by using serial sections, transmission electron microscopy, and computer-assisted image reconstruction. Fibril segments were first found in extracytoplasmic channels, the sites of their polymerization; they also were found within fibril bundles. Single fibril segments were followed over their entire length in consecutive sections, and their lengths ranged from 7 to 15 .mu.m. Structural differences in the ends of the fibril segments were identified, suggesting that the amino/carboxyl polarity of the fibril segment is reflected in its architecture. Our data indicate that fibril segments are precursors in collagen fibril formation, and we suggest that postdepositional fusion of fibril segments may be an important process in tendon development and growth.