Ischemia and reperfusion increase 125I-labeled endothelin-1 binding in rat cardiac membranes.
Ischemia and reperfusion increase 125I-labeled endothelin-1 binding in rat cardiac membranes.
复制标题
缺血和再灌注会增加大鼠心脏膜中 125I 标记的内皮素-1 的结合。
DOI：
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发表时间：
1990
影响因子：
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通讯作者：
W. Nayler
中科院分区：
文献类型：
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作者：
J. Liu;R. Chen;D. Casley;W. Nayler
以成年SD大鼠心肌膜为材料，研究了有氧灌流和再灌流对~(125)I标记猪内皮素(~(125)I-ET-1)结合部位密度(Bmax)、亲和力(Kd)和选择性的影响。非灌流心脏膜结合~(125)I-ET-1的Bmax为117.0+/-8.8fmol/mg蛋白，Kd为0.076+/-0.005 nm，希尔系数接近1。无论是有氧灌流还是常温缺血10分钟和随后的再灌流都不会改变这些结合参数。缺血发作的延长增加了Bmax(缺血20、30、60和90min分别增加23、32、62和60%)。再灌流使Bmax进一步增加(缺血20、30min再灌流15min时P<0.01，缺血60min再灌流30min时P<0.01)，但不改变选择性。缺血60min(P<0.05)和90min(P<0.01)亲和力降低。22℃的缺血对这些结合位点的密度或亲和力没有影响。
The effect of aerobic perfusion, of up to 90 min global ischemia, and of reperfusion on 125I-labeled porcine endothelin (125I-ET-1) binding site density (Bmax), affinity (KD), and selectivity was investigated using cardiac membranes harvested from adult Sprague-Dawley rats. Membranes from nonperfused hearts bound 125I-ET-1 with a Bmax of 117.0 +/- 8.8 fmol/mg protein, a KD of 0.076 +/- 0.005 nM, and a Hill coefficient approaching unity. Neither aerobic perfusion nor 10 min of normothermic ischemia and subsequent reperfusion altered these binding parameters. The extension of the ischemic episode increased Bmax (23, 32, 62, and 60% increase after 20, 30, 60, and 90 min ischemia, respectively). Reperfusion further increased Bmax (P less than 0.01 at 15 min reperfusion after 20 and 30 min ischemia, and after 30 min reperfusion following 60 min ischemia) without changing selectivity. Affinity was reduced after 60 (P less than 0.05) and 90 (P less than 0.01) min ischemia. Ischemia at 22 degrees C had no effect on the density or affinity of these binding sites.