Evaluation of enrichment method for the detection of Vibrio cholerae O1 using a rapid dipstick test in Bangladesh
Evaluation of enrichment method for the detection of Vibrio cholerae O1 using a rapid dipstick test in Bangladesh
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DOI：
10.1111/tmi.12252
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发表时间：
2014-03-01
影响因子：
3.3
通讯作者：
Alam, Munirul
中科院分区：
文献类型：
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作者：
George, Christine M.;Rashid, Mahamud-ur;Alam, Munirul
背景评价水晶VC试纸试验检测霍乱的特异性。方法采用Crystal VC试纸法和碱性蛋白水（APW）富集6 h后的细菌培养法进行比较。试纸阳性，培养阴性的样品也用PCR检测。结果收集125例患者粪便。与细菌培养相比，APW中直接检测和富集6 h后检测的总体特异性分别为91.8%和98.4% (P=0.125)，敏感性分别为65.6%和75.0% （P=0.07）。结论与直接检测相比，采用6 h富集步骤的Crystal VC试剂盒对APW的敏感性略有提高。Crystal VC试纸的特异性比先前报道的要高得多（91-98%）。因此，在资源有限的环境中，这种方法可能是一种有希望的霍乱疫情监测筛查工具，在这些环境中，消除假阳性结果至关重要。
Background To evaluate the specificity of the Crystal VC dipstick test for detecting cholera.Methods We compared direct testing using the Crystal VC dipstick test and testing after enrichment for 6 h in alkaline peptone water (APW) to bacterial culture as the gold standard. Samples positive by dipstick but negative by culture were also tested using PCR.Results Stool was collected from 125 patients. The overall specificities of the direct testing and testing after 6-h enrichment in APW compared to bacterial culture were 91.8% and 98.4% (P=0.125), respectively, and the sensitivities were 65.6% and 75.0% (P=0.07), respectively.Conclusion The increase in the sensitivity of the Crystal VC kit with the use of the 6-h enrichment step in APW compared to direct testing was marginally significant. The Crystal VC dipstick had a much higher specificity than previously reported (91-98%). Therefore, this method might be a promising screening tool for cholera outbreak surveillance in resource-limited settings where elimination of false-positive results is critical.