Upregulated long non-coding RNA LINC00152 expression is associated with progression and poor prognosis of tongue squamous cell carcinoma.
Upregulated long non-coding RNA LINC00152 expression is associated with progression and poor prognosis of tongue squamous cell carcinoma.
复制标题
长链非编码RNA LINC00152表达上调与舌鳞状细胞癌的进展和不良预后相关
DOI：
10.7150/jca.17510
复制
发表时间：
2017
影响因子：
3.9
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Yu J;Liu Y;Guo C;Zhang S;Gong Z;Tang Y;Yang L;He Y;Lian Y;Li X;Deng H;Liao Q;Li X;Li Y;Li G;Zeng Z;Xiong W;Yang X
长非编码RNA(LncRNAs)的表达改变与人类的癌变有关，可作为诊断和预后的生物标志物。然而，lncRNAs在舌鳞状细胞癌中的表达及其与舌癌诊断、进展和预后的关系尚未完全阐明。为了发现与TSCC相关的新的LncRNAs，我们分析了已发表的两组TSCC基因表达谱数据(GSE30784和GSE9844)中的LncRNA表达模式，发现LINC00152在TSCC样本中显著上调。然后，我们检测到LINC00152在另外两组TSCC样本中的表达。实时定量聚合酶链式反应(QRT-PCR)结果显示，LINC00152在15例舌鳞癌组织中高表达，而在14例癌旁舌鳞状细胞癌组织中LINC00152高表达。LINC00152的表达与舌鳞癌的T分期(p=0.009)、N分期(p=0.036)、TNM分期(p=0.017)显著相关，与复发(p<；0.001)、侵袭(p<；0.001)相关。卡普兰-迈耶分析表明，LINC00152表达的增加对舌癌患者的总体存活率(p=0.006)和无病存活率(p=0.007)都有影响。提示LINC00152有可能成为口腔鳞癌早期诊断和预后预测的潜在生物标志物。
Altered expression of long non-coding RNAs (lncRNAs) associated with human carcinogenesis and might be used as diagnosis and prognosis biomarkers. However, the expression of lncRNAs in tongue squamous cell carcinoma (TSCC) and their relevance on the diagnosis, progression and prognosis of TSCC have not been thoroughly elucidated. To discover novel TSCC-related lncRNAs, we analyzed the lncRNA expression patterns in two sets of previously published TSCC gene expression profile data (GSE30784 and GSE9844), and found that long intergenic non-coding RNA 152 (LINC00152) was significantly upregulated in TSCC samples. We then detected LINC00152 expression in two other cohorts of TSCC samples. Quantitative Real time PCR (qRT-PCR) results indicated that LINC00152 was highly expressed in 15 primary TSCC biopsies when compared with 14 adjacent non-tumor tongue squamous cell epithelium samples. The expression of LINC00152 was also measured in 182 paraffin-embedded human TSCC tissues by in situ hybridization, increased expression of LINC00152 was significantly correlated with TSCC progression, such as T stage (p = 0.009), N stage (p = 0.036), TNM stage (p = 0.017), and associated with relapse (p < 0.001), and invasion (p < 0.001). Kaplan-Meier analysis demonstrated that increased LINC00152 expression contributed to both poor overall survival (p = 0.006) and disease-free survival (p = 0.007) of TSCC patients. These findings suggest that LINC00152 might serve as a potential biomarker for early detection and prognosis prediction of TSCC.