THE INDUCIBLE IAC OPERATOR-REPRESSOR SYSTEM IS FUNCTIONAL IN MAMMALIAN-CELLS
THE INDUCIBLE IAC OPERATOR-REPRESSOR SYSTEM IS FUNCTIONAL IN MAMMALIAN-CELLS
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DOI：
10.1016/0092-8674(87)90234-0
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发表时间：
1987-02-27
期刊：
影响因子：
64.5
通讯作者：
DAVIDSON, N
中科院分区：
文献类型：
--
作者：
HU, MCT;DAVIDSON, N
我们已经研究了使用大肠杆菌乳糖操纵子-阻遏子系统来调节哺乳动物细胞中转染基因的表达。我们表明，在小鼠L细胞中产生的乳糖阻遏物通过转染的lacI表达载体块MSV-CAT融合基因的转录时，乳糖操作员插入在启动子区域内的任何一个以下位点：之间的起始密码子（ATG）和转录起始位点;之间的转录起始和TATA盒区域;或上游的TATA盒区域。这最后一个结果表明，阻遏物可能会阻止参与转录激活的蛋白质-蛋白质相互作用。诱导剂IPTG引起CAT表达的显著去抑制。乳糖阻遏物-操纵基因复合物可用作基因转移实验中基因表达调控的开/关“开关”。
We have investigated the use of the Escherichia coli lac operator-repressor system to regulate expression of transfected genes in mammalian cells. We show that lac repressor produced in mouse L cells by transfection of a lacl expression vector blocks transcription of an MSV-CAT fusion gene when the lac operator is inserted at any one of the following sites within the promoter region: between the initiation codon (ATG) and the transcription start site; between the transcription start and TATA box regions; or upstream of the TATA box region. This last result suggests that the repressor may prevent protein-protein interactions involved in transcription activation. The inducer IPTG causes a marked derepression of CAT expression. The lac repressor-operator complex may be useful as an on/off “switch” in the regulation of gene expression for gene transfer experiments.