Organophosphorous pesticide exposure alters sperm chromatin structure in Mexican agricultural workers
Organophosphorous pesticide exposure alters sperm chromatin structure in Mexican agricultural workers
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DOI：
10.1016/j.taap.2003.11.023
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发表时间：
2004-04-01
影响因子：
3.8
通讯作者：
Quintanilla-Vega, B
中科院分区：
文献类型：
--
作者：
Sánchez-Peña, LC;Reyes, BE;Quintanilla-Vega, B
我们的目的是评估职业暴露于有机磷农药混合物的男性精子染色质结构的变化，因为这些变化被认为会损害男性的生育能力和后代的发育。采用精子染色质结构测定法（SCSA）评价染色质对原位酸致变性结构的易感性。尿中烷基磷酸盐（DAP）水平被用来评估暴露。二乙基硫代磷酸（DETP）是尿液样本中发现的最常见的OP代谢物，表明主要使用硫代磷酸衍生的化合物。大多数样品的染色质结构发生了改变。约75%的精液样本被归类为生育潜力差（约占DNA碎片指数百分比[DFI%]的30%），而没有OP职业暴露的个体的平均DFI%值为9.9%。常规精液分析除存在未成熟细胞（IGC）外，大部分参数均在正常范围内，其中82%的样本高于参考值。在排除潜在混杂因素（包括IGC）后，尿DETP浓度与平均DFI和SD-DFI之间存在显著的直接关联，但与DFI%之间存在微弱关联（P = 0.079）。这表明OP暴露改变了精子染色质凝结，这可能反映在对DNA变性更敏感的细胞数量增加。这项研究表明，人类精子染色质是OP暴露的敏感靶点，可能导致不良的生殖结果。需要进一步研究蛋白磷酸化作为OP改变精子染色质的可能机制的相关性。(C) 2004爱思唯尔公司版权所有。
Our objective was to evaluate alterations in sperm chromatin structure in men occupationally exposed to a mixture of organophosphorus pesticides (OP) because these alterations have been proposed to compromise male fertility and offspring development. Chromatin susceptibility to in situ acid-induced denaturation structure was assessed by the sperm chromatin structure assay (SCSA). Urinary levels of alkylphosphates (DAP) were used to assess exposure. Diethylthiophosphate (DETP) was the most frequent OP metabolite found in urine samples indicating that compounds derived from thiophosphoric acid were mainly used. Chromatin structure was altered in most samples. About 75% of semen samples were classified as having poor fertility potential (>30% of Percentage of DNA Fragmentation Index [DFI%]), whereas individuals without OP occupational exposure showed average DFI% values of 9.9%. Most parameters of conventional semen analysis were within normality except for the presence of immature cells (IGC) in which 82% of the samples were above reference values. There were significant direct associations between urinary DETP concentrations and mean DFI and SD-DFI but marginally (P = 0.079) with DFI%, after adjustment for potential confounders, including IGC. This suggests that OP exposure alters sperm chromatin condensation, which could be reflected in an increased number of cells with greater susceptibility to DNA denaturation. This study showed that human sperm chromatin is a sensitive target to OP exposure and may contribute to adverse reproductive outcomes. Further studies on the relevance of protein phosphorylation as a possible mechanism by which OP alter sperm chromatin are required. (C) 2004 Elsevier Inc. All rights reserved.