In silico prediction and ex vivo evaluation of potential T-cell epitopes in glycoproteins 4 and 5 and nucleocapsid protein of genotype-I (European) of porcine reproductive and respiratory syndrome virus
In silico prediction and ex vivo evaluation of potential T-cell epitopes in glycoproteins 4 and 5 and nucleocapsid protein of genotype-I (European) of porcine reproductive and respiratory syndrome virus
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DOI：
10.1016/j.vaccine.2009.07.029
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发表时间：
2009-09-18
期刊：
影响因子：
5.5
通讯作者：
Mateu, Enric
中科院分区：
文献类型：
--
作者：
Diaz, Ivan;Pujols, Joan;Mateu, Enric
猪繁殖与呼吸综合征病毒（PRRSV）糖蛋白4（GP 4）、5（GP 5）和核衣壳（N）的T细胞表位使用生物信息学进行预测，随后在用改良活疫苗（MLV）或DNA（开放阅读框4、5或7）免疫的猪中通过IFN-γ ELISPOT进行测试。对于MLV免疫的猪，在N中发现了免疫显性表位，但在GP 4和GP 5中也发现了T表位。对于DNA免疫的猪，一些肽被不同地识别。使用一个大的集合的PRRSV序列，它被证明，N包含一个保守的表位和GP 5，以前报道的表位的基因型-II株的基因型-I对应物也是免疫原性的。(c)2009爱思唯尔有限公司保留所有权利。
T-cell epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) glycoproteins 4 (GP4), 5 (GP5) and nucleocapsid (N) were predicted using bioinformatics and later tested by IFN-gamma ELISPOT in pigs immunized with either a modified live vaccine (MLV) or DNA (open reading frames 4, 5 or 7). For MLV-vaccinated pigs, immunodominant epitopes were found in N but T-epitopes were also found in GP4 and GP5. For DNA-immunized pigs, some peptides were differently recognized. Using a large set of PRRSV sequences it was shown that N contains a conserved epitope and that for GP5, the genotype-I counterparts of previously reported epitopes of genotype-II strains were also immunogenic. (c) 2009 Elsevier Ltd. All rights reserved.