Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia
Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia
复制标题
DOI：
10.1128/jcm.42.5.2306-2309.2004
复制
发表时间：
2004-05-01
影响因子：
9.4
通讯作者：
Yuen, K
中科院分区：
文献类型：
--
作者：
Woo, PCY;Lau, SKP;Yuen, K
我们报告了基于重组严重急性呼吸综合征 (SARS) 冠状病毒 (SARS-CoV) 核衣壳蛋白酶联免疫吸附测定 (ELISA) 的抗体检测对 SARS-CoV 肺炎血清诊断的评估，并比较了该 ELISA 检测免疫球蛋白 G (IgG)、IgM、IgA 及其组合的敏感性和特异性，并与来自 3 年前献血的 149 名健康献血者的血清样本（作为对照）和香港有 106 名 SARS-CoV 肺炎患者。 ELISA 对 IgG、IgM 和 IgA 检测的特异性分别为 95.3、96.6 和 96.6%，相应的灵敏度分别为 94.3、59.4 和 60.4%。目前的 ELISA 似乎是 SARS-CoV 肺炎血清学诊断的敏感测试，比间接免疫荧光测定更经济且劳动强度更低，并且不需要培养 SARS-CoV。
We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobulin G (IgG), IgM, IgA, and their combinations with serum samples from 149 healthy blood donors who donated blood 3 years ago as controls and 106 SARS-CoV pneumonia patients in Hong Kong. The specificities of the ELISA for IgG, IgM, and IgA detection were 95.3, 96.6, and 96.6%, respectively, with corresponding sensitivities of 94.3, 59.4, and 60.4%, respectively. The present ELISA appears to be a sensitive test for serodiagnosis of SARS-CoV pneumonia, is much more economical and less labor-intensive than the indirect immunofluorescence assay, and does not require cultivation of SARS-CoV.