DIFFERENTIAL EXPRESSION OF HLA-D GENE-PRODUCTS IN THE NORMAL AND CELIAC SMALL BOWEL
DIFFERENTIAL EXPRESSION OF HLA-D GENE-PRODUCTS IN THE NORMAL AND CELIAC SMALL BOWEL
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DOI：
10.1111/j.1399-0039.1988.tb02076.x
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发表时间：
1988-03-01
期刊：
影响因子：
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通讯作者：
FEIGHERY, C
中科院分区：
文献类型：
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作者：
KELLY, J;WEIR, DG;FEIGHERY, C
单克隆抗体的MHC II类区域的产品，HLA-DR，DP和DQ的单态决定簇被用来研究他们的正常和腹腔（治疗和未治疗）小肠细胞上的表达。HLA-DR抗原在正常小肠中表现出特征性的分布：绒毛顶端部分的上皮细胞染色严重，并且这种染色的强度朝向绒毛基底降低。隐窝上皮细胞明显未染色。固有层内的星状细胞也呈HLA-DR阳性。HLA-DP抗原表现出类似的分布，虽然染色的强度小于所看到的HLA-DR。HLA-DQ抗原不存在于上皮细胞，仅限于固有层内的细胞。在未经治疗的腹腔疾病中，HLA-DR和HLA-DP的强度均增加，此外还观察到这些抗原的更广泛分布。除了发生在表面上皮细胞，DR和DP抗原现在存在于隐窝上皮细胞。尽管DR和DP抗原的表达发生了这种变化，但HLA-DQ的分布与正常小肠的分布基本上没有变化。治疗后的腹腔疾病的结果介于正常和未治疗的腹腔小肠之间。已经证实的正常和患病小肠上皮的II类抗原的差异表达可能对这些细胞与免疫系统细胞的相互作用有影响。
Monoclonal antibodies to monomorphic determinants of the MHC class II region products, HLA-DR, DP and DQ were used to investigate their expression on cells of the normal and coeliac (both treated and untreated) small bowel. HLA-DR antigens showed a characteristic distribution in the normal small bowel: epithelial cells in the apical portions of the villi stained heavily, and this staining decreased in intensity towards the villous base. The crypt epithelial cells were clearly unstained. Stellate cells within the lamina propria were also HLA-DR positive. HLA-DP antigens showed a similar distribution, although the intensity of staining was less than that seen with HLA-DR. HLA-DQ antigens were absent from epithelial cells and were confined solely to cells within the lamina propria. In untreated coeliac disease, the intensities of both HLA-DR and HLA-DP were increased, and in addition a more extensive distribution of these antigens was observed. In addition to occurring on the surface epithelial cells, DR and DP antigens were now present on crypt epithelial cells. Despite this change in expression of DR and DP antigens, the distribution of HLA-DQ was essentially unaltered from that of the normal small bowel. The findings in treated coeliac disease were intermediate between those in the normal and untreated coeliac small bowel. The differential expression of class II antigens by normal and diseased small bowel epithelium which has been demonstrated may have implications for interactions of these cells with cells of the immune system.