Molecular characterization of transformer-2 gene in the mud crab Scylla paramamosain and its putative role in sexual development
Molecular characterization of transformer-2 gene in the mud crab Scylla paramamosain and its putative role in sexual development
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青蟹Transformer-2基因的分子特征及其在性发育中的假定作用
DOI：
10.1111/are.14651
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发表时间：
2020
影响因子：
2
通讯作者：
Zhu Dongfa
中科院分区：
文献类型：
--
作者：
Wang Mengen;Xie Xi;Zheng Liang;Zheng Hongkun;Yu Guohong;Jin Zhongwen;Zhu Dongfa
Transformer‐2 （Tra‐2）是一种mRNA剪接因子，与Tra协同作用，调节果蝇雌性性发育。在十足动物中，由于没有发现Tra同源物，因此Tra‐2在性发育中的作用更经常被讨论。在本研究中，我们克隆并鉴定了泥crabylla paramamosain（SpTra‐2）的全长cDNA。推导出的SpTra‐2的氨基酸序列包含一个RNA识别基序（RRM），其两侧是两个富含RS的区域，这是Tra‐2蛋白的标志。esptra‐2mRNA在雄性螃蟹的肝胰腺、肌肉和雄激素腺中含量丰富，这意味着它在雄性性发育中具有潜在的作用。RNAi实验支持了这一假设，发现在体内注射sptra‐2dsRNA导致甲壳类动物雄性分化的关键基因spiag的表达降低。眼柄消融（ESA）实验进一步证实了sptra‐2和spiag2之间的关系，sptra‐2在雄激素腺和肝胰腺中的表达均早于spiag2。此外，用sptra‐2dsRNA处理也降低了spsxl的mRNA水平，但对spdmrt的表达没有影响，提示性别决定系统inS。paramamosains与印度黑腹蛇不同。
Transformer‐2 (Tra‐2) is an mRNA splicing factor that acts in concert with Tra to regulate the female sexual development inDrosophila melanogaster. In decapods, as no Tra homologues have been identified, the role of Tra‐2 in sexual development is more often discussed accordingly. In the present study, the full‐length cDNA ofTra‐2of the mud crabScylla paramamosain(SpTra‐2) was cloned and characterized. The deduced amino acid sequence of SpTra‐2 contained an RNA‐recognition motif (RRM) flanking by two RS‐rich regions, which are the hallmarks of the Tra‐2 proteins. TheSpTra‐2mRNA was highly abundant in the hepatopancreas, muscles and androgenic glands of the male crabs, implying a potential role in male sexual development. The hypothesis was supported by the RNAi experiments, finding that the injection ofSpTra‐2dsRNA in vivo led to a decreased expression ofSpIAG, a key gene in crustacean male sexual differentiation. The relationship betweenSpTra‐2andSpIAGwas further affirmed by eyestalk ablation (ESA) experiments, which causedSpTra‐2expression earlier thanSpIAGexpression in both androgenic glands and hepatopancreas. In addition, treatment withSpTra‐2dsRNA also reduced the mRNA level ofSpSxl, but had no effects onSpDmrtexpression, suggesting the sex determination system inS. paramamosainis different from that inD. melanogaster.