Spatially and temporally resolved single-cell exocytosis utilizing individually addressable carbon microelectrode arrays
Spatially and temporally resolved single-cell exocytosis utilizing individually addressable carbon microelectrode arrays
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DOI：
10.1021/ac702409s
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发表时间：
2008-03-01
影响因子：
7.4
通讯作者：
Ewing, Andrew G.
中科院分区：
文献类型：
--
作者：
Zhang, Bo;Adams, Kelly L.;Ewing, Andrew G.
我们报告的制造和表征的碳微电极阵列（MEA）和他们的应用空间和时间解决单个嗜铬细胞瘤（PC 12）细胞的神经递质释放。碳MEA由嵌入玻璃中的可单独寻址的2.5 μ m半径的微盘组成。该制造包括将每个筒中含有单个碳纤维的多管玻璃毛细管拉成尖锐的尖端，然后将电极尖端斜切以形成碳微盘阵列（10-20 μ m）。这种简单的制造过程消除了对独立电极的复杂布线的需要，从而允许制备高密度的可单独寻址的微电极。碳膜电极的特征在于使用扫描电子显微镜，稳态和快速扫描伏安法，和数值模拟。安培结果表明，单细胞胞吐的亚细胞异质性可以电化学检测与MEA。这些超小型电化学探针适用于检测狭窄空间中的快速化学事件，以及开发多功能电化学微传感器。
We report the fabrication and characterization of carbon microelectrode arrays (MEAs) and their application to spatially and temporally resolve neurotransmitter release from single pheochromocytoma (PC12) cells. The carbon MEAs are composed of individually addressable 2.5-mu m-radius microdisks embedded in glass. The fabrication involves pulling a multibarrel glass capillary containing a single carbon fiber in each barrel into a sharp tip, followed by beveling the electrode tip to form an array (10-20 mu m) of carbon microdisks. This simple fabrication procedure eliminates the need for complicated wiring of the independent electrodes, thus allowing preparation of highdensity individually addressable microelectrodes. The carbon MEAs have been characterized using scanning electron microscopy, steady-state and fast-scan voltammetry, and numerical simulations. Amperometric results show that subcellular heterogeneity in single-cell exocytosis can be electrochemically detected with MEAs. These ultrasmall electrochemical probes are suitable for detecting fast chemical events in tight, spaces, as well as for developing multifurictional electrochemical microsensors.