ROLES OF THE 2 LYSYL-TRANSFER-RNA SYNTHETASES OF ESCHERICHIA-COLI - ANALYSIS OF NUCLEOTIDE-SEQUENCES AND MUTANT BEHAVIOR
ROLES OF THE 2 LYSYL-TRANSFER-RNA SYNTHETASES OF ESCHERICHIA-COLI - ANALYSIS OF NUCLEOTIDE-SEQUENCES AND MUTANT BEHAVIOR
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DOI：
10.1128/jb.172.6.3237-3243.1990
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发表时间：
1990-06-01
影响因子：
3.2
通讯作者：
NEIDHARDT, FC
中科院分区：
文献类型：
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作者：
CLARK, RL;NEIDHARDT, FC
测定了大肠杆菌热诱导赖氨酰-tRNA合成酶基因lysU的完整核苷酸序列，并与组成型赖氨酰-tRNA合成酶基因lysS（herC）的已发表序列进行了比较。发现这些不连锁的基因在其长度的72%以上是相同的。LysU和LysS基因产物的氨基酸序列同源性在85%以上，相似性在92%以上。在多拷贝质粒上携带lysU野生型等位基因的菌株生长期间高水平LysU的积累对生长或LysS的合成没有可观察到的影响。构建lysU缺失菌株，并显示其在低温（28 ℃）下正常生长。C）但在44度时很差。C;在高温下缓慢生长（正常的45%）被携带野生型lysU的质粒完全逆转。这些研究结果的存在的两个氨酰-tRNA合成酶赖氨酸的影响进行了讨论。
The complete nucleotide sequence of lysU, the gene for the heat-inducible lysyl-tRNA synthetase of Escherichia coli, was determined and compared with the published sequence of lysS (herC), the gene for the constitutive lysyl-tRNA synthetase. These unlinked genes were found to be identical over 72% of their lengths. the deduced amino acid sequences of the respective gene products, LysU and LysS, were identical over 85% and similar over 92% of their lengths. Accumulation of high levels of LysU during growth of strains carrying the wild-type allele of lysU on multicopy plasmids had no observable effect on growth or on the synthesis of LysS. A lysU deletion strain was constructed and was shown to grow normally at low temperature (28.degree. C) but poorly at 44.degree. C; the slow growth (45% of normal) at elevated temperature was fully reversed by plasmids bearing wild-type lysU. The implications of these findings for the existence of two aminoacyl-tRNA synthetases for lysine are discussed.