Morphology and adhesion of mesenchymal stem cells on PLLA, apatite and apatite/collagen surfaces
Morphology and adhesion of mesenchymal stem cells on PLLA, apatite and apatite/collagen surfaces
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DOI：
10.1007/s10856-007-3195-2
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发表时间：
2008-07-01
影响因子：
3.7
通讯作者：
Sun, S.
中科院分区：
文献类型：
--
作者：
Chen, Yun;Cho, M. R.;Sun, S.
本研究对仿生磷灰石/胶原复合涂层的制备、表征及其与成骨样细胞的相互作用进行了研究，以了解人骨髓间充质干细胞(HMSC)对该表面的反应。比较了PLLA膜、磷灰石涂层的PLLA膜和磷灰石/胶原复合涂层的PLLA膜。首次用荧光显微镜观察了hMSC在这种条件下的形态。为了在分子水平上进一步了解这种细胞-材料的相互作用，研究了整合素的表达、肌动蛋白的组装和纽蛋白阳性的焦点黏附斑块。结果表明，在三种表面中，干细胞在磷灰石/胶原复合材料表面的铺展效果最好，其次是磷灰石表面，最后是PLLA对照组。整合素在磷灰石/胶原表面的表达高于在磷灰石和PLLA表面的表达。纽蛋白和肌动蛋白的免疫染色表明，PLLA上的复合涂层增强了局灶性粘连的形成。
Biomimetic apatite/collagen composite coating, previously reported particularly with regard to its fabrication, characterization and interaction with osteoblast-like cells, has been investigated in this study to understand the response of human mesenchymal stem cells (hMSC) to such surface. PLLA films and PLLA films with apatite coating were compared with PLLA films with apatite/collagen composite coating. The hMSC morphology in response to such conditions was first observed using fluorescence microscopy. To further understand such cell-material interactions at a molecular level, integrin expression, actin assembly and vinculin-positive focal adhesion plaques were examined. Our results demonstrated that spreading of stem cells on the apatite/collagen composite surface was determined best among the three types of surfaces, followed by the apatite surface and then the PLLA control. Integrin expression on the apatite/collagen surface was higher than those on the apatite surface and PLLA surface. Immunostaining for vinculin and actin suggested that the composite coating on PLLA enhanced the formation of focal adhesion.