Neuropeptidomics: Mass Spectrometry-Based Identification and Quantitation of Neuropeptides.
Neuropeptidomics: Mass Spectrometry-Based Identification and Quantitation of Neuropeptides.
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DOI：
10.5808/gi.2016.14.1.12
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发表时间：
2016-03
影响因子：
--
通讯作者：
Lee JE
中科院分区：
文献类型：
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作者：
Lee JE
由前激素通过内肽酶的选择性作用产生的神经肽是重要的信号分子，在成瘾、抑郁、疼痛和昼夜节律等多种生理过程中起着关键作用。神经肽与突触后受体结合，并像经典的神经递质一样引发细胞效应。虽然每种神经肽都有其自身的生物学功能，但质谱（MS）允许在没有先验知识的情况下鉴定每种肽的精确分子形式，并在不同条件下对样品中的神经肽进行定量。在过去的十年中，基于ms的神经肽组学方法已经应用于各种动物模型和条件来表征和量化新的神经肽，以及已知的神经肽，促进了我们对神经系统功能的理解。在这里，我们将介绍神经肽的概述和基于ms的神经肽组学策略，用于鉴定和定量神经肽。
Neuropeptides produced from prohormones by selective action of endopeptidases are vital signaling molecules, playing a critical role in a variety of physiological processes, such as addiction, depression, pain, and circadian rhythms. Neuropeptides bind to post-synaptic receptors and elicit cellular effects like classical neurotransmitters. While each neuropeptide could have its own biological function, mass spectrometry (MS) allows for the identification of the precise molecular forms of each peptide without a priori knowledge of the peptide identity and for the quantitation of neuropeptides in different conditions of the samples. MS-based neuropeptidomics approaches have been applied to various animal models and conditions to characterize and quantify novel neuropeptides, as well as known neuropeptides, advancing our understanding of nervous system function over the past decade. Here, we will present an overview of neuropeptides and MS-based neuropeptidomic strategies for the identification and quantitation of neuropeptides.