Studies on principal components and antioxidant activity of different Radix Astragali samples using high-performance liquid chromatography/electrospray ionization multiple-stage tandem mass spectrometry
Studies on principal components and antioxidant activity of different Radix Astragali samples using high-performance liquid chromatography/electrospray ionization multiple-stage tandem mass spectrometry
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高效液相色谱/电喷雾多级串联质谱法研究不同黄芪样品的主要成分及抗氧化活性
DOI：
10.1016/j.talanta.2009.01.021
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发表时间：
2009-05-15
期刊：
影响因子：
6.1
通讯作者：
Liu, Shuying
中科院分区：
文献类型：
--
作者：
Huang, Xin;Liu, Yan;Liu, Shuying
采用高效液相色谱-电喷雾电离离子阱多级串联质谱(HPLC-ESI-IT-MSn)方法对黄芪提取物中的主要成分异黄酮和黄芪甲苷进行检测。通过将HPLC的保留时间(t(R))、ESI-MSn数据和分析化合物的结构与参比化合物和文献中的数据进行比较，已鉴定或初步推导了17种异黄酮和12种黄芪甲苷。利用提取离子色谱(EIC)模式，当不同成分形成重叠峰时，可以实现异黄酮和黄芪甲苷的同时测定。并利用该方法对和龙市不同生长年限的黄芪提取物进行了成分分析。然后采用多选离子监测（MIM）模式下的HPLC-ESI-MS方法，应用自旋捕获技术成功研究了不同样品的抗氧化活性，并应用铁还原抗氧化能力（FRAP）测定来支持结果。总结了黄芪中异黄酮、黄芪甲苷成分与抗氧化活性的相关性。本文表明，HPLC-ESI-MSn 是表征中药主要成分和评价中药抗氧化活性的有力方法。 (c) 2009 Elsevier B.V. 保留所有权利。
The principal components, isoflavonoids and astragalosides, in the extract of Radix Astragali were detected by a high-performance liquid chromatography Couple to electrospray ionization ion trap multiple-stage tandem mass spectrometry (HPLC-ESI-IT-MSn) method. By comparing the retention time (t(R)) of HPLC, the ESI-MSn data and the structures of analyzed Compounds with the data of reference compounds and in the literature, 17 isoflavonoids and 12 astragalosides have been identified or tentatively deduced. By Virtue of the extracted ion chromatogram (EIC) mode, simultaneous determination of isoflavonoids and astragalosides could be achieved when the different components formed overlapped peaks. And this method has been utilized to analyze the constituents in extracts of Radix Astragali from Helong City and of different growth years. Then the antioxidant activity of different samples has been Successfully investigated by HPLC-ESI-MS method in multiple selected ion monitoring(MIM) mode, applying the spin trapping technology, and the Ferric Reducing Antioxidant Power (FRAP) assay was applied to support the result. The correlations of the isoflavonoids and astragalosides components and the antioxidant activities of Radix Astragali were summarized. The present paper demonstrates that HPLC-ESI-MSn is a powerful method for the characterization of the principal components and evaluation of the antioxidant activity of Chinese medicinal herbs. (c) 2009 Elsevier B.V. All rights reserved.