CHARACTERIZATION OF A CATALASE-DEFICIENT STRAIN OF NEISSERIA-GONORRHOEAE - EVIDENCE FOR THE SIGNIFICANCE OF CATALASE IN THE BIOLOGY OF N-GONORRHEA
CHARACTERIZATION OF A CATALASE-DEFICIENT STRAIN OF NEISSERIA-GONORRHOEAE - EVIDENCE FOR THE SIGNIFICANCE OF CATALASE IN THE BIOLOGY OF N-GONORRHEA
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DOI：
10.1128/iai.61.4.1232-1238.1993
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发表时间：
1993-04-01
影响因子：
3.1
通讯作者：
ARKO, RJ
中科院分区：
文献类型：
--
作者：
JOHNSON, SR;STEINER, BM;ARKO, RJ
我们获得了一个过氧化氢酶缺陷（Kat-）菌株淋病奈瑟菌分离的病人谁是青霉素治疗失败。定量酶分析和电泳的细胞提取物上的天然聚丙烯酰胺凝胶随后染色过氧化氢酶和过氧化物酶的活动未能检测到这两种酶。在普通实验室条件下生长时，该菌株未表现出生长异常或异常要求。然而，Kat-菌株被证明对外源性过氧化氢极其敏感，并且在这样的暴露之后对细菌DNA的分析显示染色体和质粒DNA中的广泛单链断裂。淋球菌过氧化氢酶从Kat+实验室菌株的部分表征表明，该酶的物理和化学性质的过氧化氢酶和过氧化物酶。
We obtained a catalase-deficient (Kat-) strain of Neisseria gonorrhoeae isolated from a patient who had been unsuccessfully treated with penicillin. Quantitative enzyme assays and electrophoresis of cell extracts on native polyacrylamide gels subsequently stained for catalase and peroxidase activities failed to detect both enzymes. The strain exhibited no growth anomalies or unusual requirements when grown under ordinary laboratory conditions. However, the Kat- strain proved extremely sensitive to exogenous hydrogen peroxide, and analysis of the bacterial DNA after such exposure showed extensive single-strand breakage in both chromosomal and plasmid DNAs. Partial characterization of the gonococcal catalase from a Kat+ laboratory strain revealed that the enzyme had the physical and chemical properties of both catalase and peroxidase.