Cytotoxic activity of flavonoids from the flowers of Chrysanthemum morifolium on human colon cancer Colon205 cells
Cytotoxic activity of flavonoids from the flowers of Chrysanthemum morifolium on human colon cancer Colon205 cells
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DOI：
10.1080/10286020903128470
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发表时间：
2009-01-01
影响因子：
1.7
通讯作者：
Wu, Chun-Fu
中科院分区：
文献类型：
--
作者：
Xie, Yuan-Yuan;Yuan, Dan;Wu, Chun-Fu
从菊花中分离得到一个新的对羟基苯乙酰黄酮类化合物Diosmetin 7-(6‘’-O-p-hydroxyphenylacetyl)O-beta-D-glucopyranoside(1))。《怀菊》cv.11月(菊科)，以及五个已知的黄酮类化合物，木犀草素(2)，洋地黄素(3)，洋地黄素7-O-β-D-吡喃葡萄糖苷(4)，洋地黄素(5)和头孢菌素(6)，以及四个已知的咖啡酰奎宁酸衍生物，即马钱子素F(7)，3，5-二咖啡酰奎宁酸(8)，1，3-二咖啡酰基-表奎宁酸(9)和绿原酸(10)。1的结构经UV、IR、ESI-TOF-MS、一维和二维核磁共振波谱确证。采用四甲基偶氮唑盐比色法测定化合物1-5对人结肠癌细胞株COLON205的细胞毒活性。化合物2和3对COLON205有明显的细胞毒作用，IC50值分别为96.9和82.9微米。然而，化合物1、4和5几乎没有细胞毒活性。
A new p-hydroxyphenylacetyl flavonoid, diosmetin 7-(6 ''-O-p-hydroxyphenylacetyl)O-beta-D-glucopyranoside (1), was isolated from the flowers of Chrysanthemum morifolium Ramat. 'huaiju' cv. nov. (Compositae), together with five known flavonoids, luteolin (2), diosmetin (3), diosmetin 7-O-beta-D-glucopyranoside (4), diosmin (5), and scolimoside (6), and four known caffeoylquinic acid derivatives, macranthoin F (7), 3,5-dicaffeoylquinic acid (8), 1,3-dicaffeoyl-epi-quinic acid (9), and chlorogenic acid (10). The structure of 1 was elucidated by UV, IR, ESI-TOF-MS, 1D, and 2D NMR spectroscopic methods. Cytotoxic activity of compounds 1-5 against human colon cancer cell Colon205 was investigated using MTT assays. Compounds 2 and 3 showed significant cytotoxicities against Colon205, with their IC50 values being 96.9 and 82.9 mu M, respectively. However, compounds 1, 4, and 5 showed little cytotoxic activity.