DNA replication events during larval silk gland development in the silkworm, Bombyx mori.
DNA replication events during larval silk gland development in the silkworm, Bombyx mori.
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DOI：
10.1016/j.jinsphys.2012.04.017
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发表时间：
2012-07
影响因子：
2.2
通讯作者：
Chun-Dong Zhang;Fang Li;Xiangyun Chen;Mao-Hua Huang;Jun Zhang;H. Cui;M. Pan;Cheng Lu
中科院分区：
文献类型：
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作者：
Chun-Dong Zhang;Fang Li;Xiangyun Chen;Mao-Hua Huang;Jun Zhang;H. Cui;M. Pan;Cheng Lu
蚕丝腺是合成蚕丝蛋白的重要器官，对纺丝起着至关重要的作用。蚕丝腺细胞的基因组DNA含量在幼虫的生命周期中急剧增加20 - 40万倍。在离体培养中，用BrdU标记法测定了幼虫蜕皮期和蜕皮间隔期的DNA合成。我们发现，细胞周期在蜕皮间期被激活，而在蜕皮期被抑制。5龄幼虫的前丝腺、中丝腺和后丝腺细胞在第6天时异步退出胞内分裂周期，这与徘徊期细胞周期相关的cdt1、pcna、cyclin E、cdk2和cdk1 mrna的表达减少有关。额外的饥饿对新蜕皮幼虫丝腺DNA合成的启动没有影响。
The silk gland is an important organ in silkworm as it synthesizes silk proteins and is critical to spinning. The genomic DNA content of silk gland cells dramatically increases 200–400 thousand times for the larval life span through the process of endomitosis. Using in vitro culture, DNA synthesis was measured using BrdU labeling during the larval molt and intermolt periods. We found that the cell cycle of endomitosis was activated during the intermolt and was inhibited during the molt phase. The anterior silk gland, middle silk gland, and posterior silk gland cells asynchronously exit the endomitotic cycle after day 6 in 5th instar larvae, which correlated with the reduced expression of the cell cycle-related cdt1, pcna, cyclin E, cdk2 and cdk1 mRNAs in the wandering phase. Additional starvation had no effect on the initiation of silk gland DNA synthesis of the freshly ecdysed larvae.