INTRACELLULAR-TRANSPORT OF MICRO-INJECTED 5S AND SMALL NUCLEAR RNAS
INTRACELLULAR-TRANSPORT OF MICRO-INJECTED 5S AND SMALL NUCLEAR RNAS
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DOI：
10.1038/295572a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
PARISOT, RF
中科院分区：
文献类型：
--
作者：
DEROBERTIS, EM;LIENHARD, S;PARISOT, RF
本文用~（32）P标记的HeLa细胞总RNA显微注射到异种卵母细胞的胞质中，分析了RNA在细胞核中的分离机制。小的核RNA（U1、U2、U4、U 5和U6）迁移到细胞核中，在那里它们变得比在细胞质中浓缩30-60倍。其他RNA，如tRNA和7S RNA，留在细胞质中，而5S RNA则集中在核仁中。对红斑狼疮抗体的研究表明，迁移的RNA与卵母细胞RNA结合蛋白相关。
The mechanism by which some RNAs are segregated in the cell nucleus was analysed by microinjecting32P-labelled total RNA from HeLa cells into the cytoplasm ofXenopusoocytes. Small nuclear RNAs (U1, U2, U4, U5 and U6) migrated into the cell nucleus, where they became 30–60 fold more concentrated than in the cytoplasm. Other RNAs, such as tRNA and 7S RNA, remained in the cytoplasm, while 5S RNA became concentrated in the nucleolus. Studies with lupus erythematosus antibodies showed that the migrating RNAs become associated with oocyte RNA-binding proteins.