Comprehensive analysis of resistance-nodulation-cell division superfamily (RND) efflux pumps from Serratia marcescens, Db10.
Comprehensive analysis of resistance-nodulation-cell division superfamily (RND) efflux pumps from Serratia marcescens, Db10.
复制标题
对粘质沙雷氏菌 Db10 的抗性结瘤细胞分裂超家族 (RND) 外排泵进行综合分析。
DOI：
10.1038/s41598-019-41237-7
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Kuroda T.
中科院分区：
文献类型：
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作者：
Toba S;Minato Y;Kondo Y;Hoshikawa K;Minagawa S;Komaki S;Kumagai T;Matoba Y;Morita D;Ogawa W;Gotoh N;Tsuchiya T;Kuroda T.
我们研究了粘质沙雷氏菌耐药-增殖-细胞分裂超家族（RND）外排系统在其内在多药耐药中的作用。我们确定了8个假定的RND外排系统基因在thes.marcescensDb10基因组，包括以前的特点系统，sdeXY，sdeAB，和sdeCDE。在这8个基因中，有6个基因使大肠杆菌KAM 32产生多药耐药。八个基因中的五个赋予苯扎氯铵抗性，表明RND外排系统在粘质沙雷氏菌抗生物杀灭剂中的重要性。使用罗丹明6 G外排试验检查了5个泵的能量依赖性外排活性。当在大肠杆菌的tolC缺陷菌株KAM 43中表达时，没有一个基因赋予大肠杆菌抗性。当编码粘质沙雷氏菌TolC直系同源物的HasF在KAM 43中表达时，所有基因都赋予大肠杆菌抗性，表明HasF是该生物体中所有RND外排系统所使用的主要外膜蛋白。我们从临床分离的多重耐药粘质沙雷氏菌衍生株中构建了deXY缺失突变株，发现该deXY缺失突变株对广谱抗菌药物敏感。
We investigated the role of the resistance-nodulation-cell division superfamily (RND) efflux system on intrinsic multidrug resistance inSerratia marcescens. We identified eight putative RND efflux system genes in theS.marcescensDb10 genome that included the previously characterized systems,sdeXY,sdeAB, andsdeCDE. Six out of the eight genes conferred multidrug resistance on KAM32, a drug hypersensitive strain ofEscherichia coli. Five out of the eight genes conferred resistance to benzalkonium, suggesting the importance of RND efflux systems in biocide resistance inS.marcescens. The energy-dependent efflux activities of five of the pumps were examined using a rhodamine 6 G efflux assay. When expressed in thetolC-deficient strain ofE.coli, KAM43, none of the genes conferred resistance onE.coli. WhenhasF, encoding theS.marcescensTolC ortholog, was expressed in KAM43, all of the genes conferred resistance onE.coli, suggesting that HasF is a major outer membrane protein that is used by all RND efflux systems in this organism. We constructed asdeXYdeletion mutant from a derivative strain of the clinically isolated multidrug-resistantS.marcescensstrain and found that thesdeXYdeletion mutant was sensitive to a broad spectrum of antimicrobial agents.