INFECTIOUS RNA DERIVED BY TRANSCRIPTION FROM CLONED CDNA COPIES OF THE GENOMIC RNA OF AN INSECT VIRUS
INFECTIOUS RNA DERIVED BY TRANSCRIPTION FROM CLONED CDNA COPIES OF THE GENOMIC RNA OF AN INSECT VIRUS
复制标题
DOI：
10.1073/pnas.83.1.63
复制
发表时间：
1986-01-01
影响因子：
11.1
通讯作者：
KAESBERG, P
中科院分区：
文献类型：
--
作者：
DASMAHAPATRA, B;DASGUPTA, R;KAESBERG, P
BBV（黑甲虫病毒）基因组 RNA 的克隆 cDNA 的 RNA 转录物对培养的黑腹果蝇细胞具有感染性。单个转录本的感染性约为相应真实病毒粒子 RNA 的 10%。根据蔗糖密度梯度沉降、特异性感染性、噬斑形态和血清学判断，由转录物感染产生的子代病毒在表型上与用于产生原始cDNA形式的祖病毒没有区别。尽管用于产生该病毒的转录RNA具有20个非病毒碱基，其5''末端有一个加帽基团，但这20个碱基在子代病毒RNA中不存在。 cDNA形式以及由此产生的转录RNA应该可以容易地通过重组DNA技术进行修饰，以用于病毒研究以及将外源基因插入到病毒基因组中，从而插入到宿主细胞质中。
RNA transcripts of cloned cDNA of the genomic RNAs of BBV (black beetle virus) are infectious to cultured cells of Drosophila melanogaster. Individual transcripts had approximately 10% of the infectivity of the corresponding authentic virion RNA. Progeny virus resulting from transcript infection was phenotypically indistinguishable from the progenitor virus used to generate the original cDNA forms as judged by sucrose density gradient sedimentation, specific infectivity, plaque morphology, and serology. Although the transcript RNAs used to produce this virus had 20 nonviral bases headed by a capping group at their 5'' termini, these 20 bases were absent in the progeny viral RNAs. The cDNA forms, and therefore the resulting transcript RNAs, should be readily modifiable by the techniques for recombinant DNA technology both for viral studies and for the insertion of foreign genes into the viral genome and thus into the host cytoplasm.