Zirconia based nucleic acid sensor for Mycobacterium tuberculosis detection
Zirconia based nucleic acid sensor for Mycobacterium tuberculosis detection
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DOI：
10.1063/1.3293447
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发表时间：
2010-03-29
影响因子：
4
通讯作者：
Malhotra, B. D.
中科院分区：
文献类型：
--
作者：
Das, Maumita;Sumana, Gajjala;Malhotra, B. D.
采用电化学方法在金（Au）表面沉积纳米氧化锆（ZrO 2）薄膜（粒径约35 nm），利用磷酸基上的氧原子与锆的亲和性，制备了结核分枝杆菌特异性21聚体寡核苷酸探针（ssDNA）的DNA生物传感器。该DNA-ZrO 2/Au电极可用于M. 60 s内检出限为0.065 ng/μ L。
Nanostructured zirconium oxide (ZrO2) film (particle size similar to 35 nm), electrochemically deposited onto gold(Au) surface, has been used to immobilize 21-mer oligonucleotide probe (ssDNA) specific to Mycobacterium tuberculosis by utilizing affinity between oxygen atom of phosphoric group and zirconium to fabricate DNA biosensor. This DNA-ZrO2/Au bioelectrode, characterized using x-ray diffraction, Fourier transform infrared spectroscopy, cyclic voltammetry, and scanning electron microscopy techniques, can be used for early and rapid diagnosis of M. tuberculosis with detection limit of 0.065 ng/mu L within 60s.