Transcriptomic Analysis of the Innate Antiviral Immune Response in Porcine Intestinal Epithelial Cells: Influence of Immunobiotic Lactobacilli.
Transcriptomic Analysis of the Innate Antiviral Immune Response in Porcine Intestinal Epithelial Cells: Influence of Immunobiotic Lactobacilli.
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DOI：
10.3389/fimmu.2017.00057
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发表时间：
2017
影响因子：
7.3
通讯作者：
Villena J
中科院分区：
文献类型：
--
作者：
Albarracin L;Kobayashi H;Iida H;Sato N;Nochi T;Aso H;Salva S;Alvarez S;Kitazawa H;Villena J
鼠李糖乳杆菌CRL1505和植物乳杆菌CRL1506是免疫生物菌株，能够增强对病毒性肠道感染的保护作用，这在动物模型和人类中得到了证明。为了深入了解宿主与免疫生物的相互作用，本研究研究了猪肠上皮细胞（PIE）对病毒分子相关模式poly（I:C）攻击的转录组反应，以及免疫生物菌株CRL1505和CRL1506诱导的转录组谱变化。通过使用微阵列技术和反转录PCR，我们获得了PIE细胞中参与先天抗病毒免疫反应的免疫基因的整体概述。poly（I:C）刺激PIE细胞显著增加IFN-α和IFN-β、几种干扰素刺激基因、细胞因子、趋化因子、粘附分子和参与前列腺素生物合成的基因的表达。乳酸菌对poly（I:C）诱导的PIE细胞免疫基因表达有不同的调节作用。最显著的变化发现抗病毒因子（IFN-α、IFN-β、NPLR3、OAS1、OASL、MX2和RNASEL）和细胞因子/趋化因子（IL-1β、IL-6、CCL4、CCL5和CXCL10）在乳杆菌处理的PIE细胞中显著升高。免疫制剂可降低介导多（I:C）炎症损伤的IL-15和RAE1基因的表达。此外，乳酸菌处理增加了参与前列腺素E2生物合成的PLA2G4A、PTGES和PTGS2的表达。L. rhamnosus CRL1505和L. plantarum CRL1506在调节PIE细胞先天抗病毒免疫反应的能力上表现出数量和质量上的差异，这可以解释CRL1505菌株比CRL1506在体内保护病毒感染和炎症损伤的能力更高。这些结果为深入了解宿主-免疫生物相互作用及其对抗病毒免疫的影响提供了有价值的信息。综合转录组学分析成功鉴定出一组基因（IFN-β、RIG1、RNASEL、MX2、A20、IL27、CXCL5、CCL4、PTGES和PTGER4），这些基因可作为PIE细胞中筛选新的抗病毒免疫生物和开发新型功能食品和饲料的前瞻性生物标志物，可能有助于预防病毒感染。
Lactobacillus rhamnosus CRL1505 and Lactobacillus plantarum CRL1506 are immunobiotic strains able to increase protection against viral intestinal infections as demonstrated in animal models and humans. To gain insight into the host–immunobiotic interaction, the transcriptomic response of porcine intestinal epithelial (PIE) cells to the challenge with viral molecular associated pattern poly(I:C) and the changes in the transcriptomic profile induced by the immunobiotics strains CRL1505 and CRL1506 were investigated in this work. By using microarray technology and reverse transcription PCR, we obtained a global overview of the immune genes involved in the innate antiviral immune response in PIE cells. Stimulation of PIE cells with poly(I:C) significantly increased the expression of IFN-α and IFN-β, several interferon-stimulated genes, cytokines, chemokines, adhesion molecules, and genes involved in prostaglandin biosynthesis. It was also determined that lactobacilli differently modulated immune gene expression in poly(I:C)-challenged PIE cells. Most notable changes were found in antiviral factors (IFN-α, IFN-β, NPLR3, OAS1, OASL, MX2, and RNASEL) and cytokines/chemokines (IL-1β, IL-6, CCL4, CCL5, and CXCL10) that were significantly increased in lactobacilli-treated PIE cells. Immunobiotics reduced the expression of IL-15 and RAE1 genes that mediate poly(I:C) inflammatory damage. In addition, lactobacilli treatments increased the expression PLA2G4A, PTGES, and PTGS2 that are involved in prostaglandin E2 biosynthesis. L. rhamnosus CRL1505 and L. plantarum CRL1506 showed quantitative and qualitative differences in their capacities to modulate the innate antiviral immune response in PIE cells, which would explain the higher capacity of the CRL1505 strain when compared to CRL1506 to protect against viral infection and inflammatory damage in vivo. These results provided valuable information for the deeper understanding of the host–immunobiotic interaction and their effect on antiviral immunity. The comprehensive transcriptomic analyses successfully identified a group of genes (IFN-β, RIG1, RNASEL, MX2, A20, IL27, CXCL5, CCL4, PTGES, and PTGER4), which can be used as prospective biomarkers for the screening of new antiviral immunobiotics in PIE cells and for the development of novel functional food and feeds, which may help to prevent viral infections.