An Amperometric Glucose Sensor Integrated into an Insulin Delivery Cannula: In Vitro and In Vivo Evaluation.
An Amperometric Glucose Sensor Integrated into an Insulin Delivery Cannula: In Vitro and In Vivo Evaluation.
复制标题
集成到胰岛素输送插管中的电流式葡萄糖传感器：体外和体内评估。
DOI：
10.1089/dia.2016.0407
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发表时间：
2017
影响因子：
5.4
通讯作者：
Cargill,RobertS
中科院分区：
文献类型：
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作者：
Ward,WKenneth;Heinrich,Gabriel;Breen,Matthew;Benware,Sheila;Vollum,Nicole;Morris,Kristin;Knutsen,Chad;Kowalski,JosephD;Campbell,Scott;Biehler,Jerry;Vreeke,MarkS;Vanderwerf,ScottM;Castle,JessicaR;Cargill,RobertS
背景：标签禁止在皮下连续血糖监测 (CGM) 部位输送胰岛素。将传感和胰岛素输送功能集成到单个设备中可能会增加 CGM 在 1 型糖尿病患者中的使用。方法：为了了解这种干扰的性质，我们使用层压到胰岛素输送套管外壁的柔性电极条测量了猪推注胰岛素输送部位的葡萄糖。在传感设计方面，我们将偏置为 600 mV 的 H2O2 测量传感器与偏置为 175 mV 的氧化还原介体型传感器进行了比较。结果：在 H2O2 测量传感器中，但在氧化还原介导化学传感器中，在胰岛素 lis-pro 推注后观察到电流的虚假上升。这种长时间的伪影伴随着电极中毒。在氧化还原介导的传感器中，在输送盐水和没有任何液体输送期间获得的传感器信号模式与在胰岛素输送期间获得的传感器信号模式相似。 结论：综合考虑体外和体内研究结果，很明显，干扰机制是胰岛素制剂中存在的酚类防腐剂在高偏压下的氧化。通过使用低偏压的氧化还原介体化学可以避免这种效应。
Background:Labeling prohibits delivery of insulin at the site of subcutaneous continuous glucose monitoring (CGM). Integration of the sensing and insulin delivery functions into a single device would likely increase the usage of CGM in persons with type 1 diabetes.Methods:To understand the nature of such interference, we measured glucose at the site of bolus insulin delivery in swine using a flexible electrode strip that was laminated to the outer wall of an insulin delivery cannula. In terms of sensing design, we compared H2O2-measuring sensors biased at 600 mV with redox mediator-type sensors biased at 175 mV.Results:In H2O2-measuring sensors, but not in sensors with redox-mediated chemistry, a spurious rise in current was seen after insulin lis-pro boluses. This prolonged artifact was accompanied by electrode poisoning. In redox-mediated sensors, the patterns of sensor signals acquired during delivery of saline and without any liquid delivery were similar to those acquired during insulin delivery.Conclusion:Considering in vitro and in vivo findings together, it became clear that the mechanism of interference is the oxidation, at high bias potentials, of phenolic preservatives present in insulin formulations. This effect can be avoided by the use of redox mediator chemistry using a low bias potential.