Glycoprotein G deletion mutants of equine herpesvirus 1 (EHV1; equine abortion virus) and EHV4 (equine rhinopneumonitis virus)
Glycoprotein G deletion mutants of equine herpesvirus 1 (EHV1; equine abortion virus) and EHV4 (equine rhinopneumonitis virus)
复制标题
马疱疹病毒 1（EHV1；马流产病毒）和 EHV4（马鼻肺炎病毒）的糖蛋白 G 缺失突变体
作者：
J. Huang;C. Hartley;N. Ficorilli;B. Crabb;M. Studdert
总结：构建了EHV 1和EHV 4的糖蛋白G（gG）缺失突变体，命名为EHV 1 ΔgG和EHV 4 ΔgG。EHV 1 ΔgG突变株的生长特性与亲本病毒相似。所有的EHV 4 ΔgG突变体在细胞培养中生长更慢，并产生不同形态的空斑，包括更小的尺寸。两种gG缺失突变体病毒在细胞培养物中的产量与亲本病毒相似。对突变体病毒gG侧翼基因测序、Southern blot、PCR和western blot分析表明，除EHV 4 ΔgG突变体外，其缺失与预期一致，该突变体除了gG缺失外，还包含相邻下游基因ORF 71（糖蛋白2）的意外缺失。EHV 1 ΔgG和EHV 4 ΔgG的抗血清中和相应的突变体和亲本病毒至相同滴度，并且这些抗血清可以在gG抗体检测ELISA中与野生型病毒的抗血清区分开。突变病毒可用作疫苗候选物，并且gG的缺失可作为区分接种疫苗的马与自然感染的马的标记。
Summary.Glycoprotein G (gG) deletion mutants of EHV1 and EHV4, designated EHV1ΔgG and EHV4ΔgG, were constructed. The growth characteristics of the EHV1ΔgG mutants were similar to the parent virus. All of the EHV4ΔgG mutants grew more slowly in cell culture and produced plaques of different morphology including smaller size. The yields of both gG deletion mutant viruses in cell culture were similar to the parent viruses. Sequencing of the genes flanking gG, Southern blot, PCR and western blot analyses of the mutant viruses demonstrated that the deletions were as expected, except for EHV4ΔgG mutants, which in addition to deletion of gG contained unexpected deletions in the adjacent down stream gene ORF 71 (glycoprotein 2). Antisera to EHV1ΔgG and EHV4ΔgG neutralised the respective mutant and the parent viruses to the same titre and these antisera could be distinguished from antisera to the wild type viruses in a gG antibody detection ELISA. The mutant viruses may be useful as vaccine candidates and the deletion of gG may act as a marker to distinguish vaccinated from the naturally infected horses.