Synthesis, Characterization and Antibacterial Activity of Chlorophyllin Functionalized Graphene Oxide Nanostructures
Synthesis, Characterization and Antibacterial Activity of Chlorophyllin Functionalized Graphene Oxide Nanostructures
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DOI：
10.1166/sam.2014.1767
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发表时间：
2014-04-01
影响因子：
0.9
通讯作者：
Karimnezhad, Hanieh
中科院分区：
文献类型：
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作者：
Azimi, Sara;Behin, Jamshid;Karimnezhad, Hanieh
为了增强氧化石墨烯对大肠杆菌的抗菌效果，采用水溶性叶绿素和锌金属离子制备了两种功能化氧化石墨烯-叶绿素和氧化石墨烯-叶绿素-锌纳米材料。功能化的氧化石墨烯，其特征在于使用傅里叶变换红外光谱，能量色散X射线光谱，紫外-可见光谱，和场发射扫描电子显微镜。在相同浓度和培养条件下，氧化石墨烯-叶绿酸-锌分散体的抗菌活性最高，其次是氧化石墨烯-叶绿酸和氧化石墨烯。细菌细胞膜的变化也进行了研究，通过场发射扫描电子显微镜。细菌与官能化氧化石墨烯纳米材料的孵育导致细胞完整性的丧失，并因此导致细胞死亡。结果表明，表面化学和金属毒性在使氧化石墨烯-叶绿酸-Zn成为强抗菌剂中起主要作用。提出了功能化氧化石墨烯纳米材料的抗菌机理。
To enhance the antimicrobial effect of graphene oxide against Escherichia coli, water soluble chlorophyllin and zinc metal ions were used to prepare two types of functionalized GO nanonnaterials, namely, graphene oxide-chlorophyllin and graphene oxide-chlorophyllin-Zn. Functionalized graphene oxide were characterized using Fourier transform infrared spectroscopy, energy dispersive X-ray spectroscopy, ultra violet-visible spectroscopy, and field emission scanning electron microscopy. Under similar concentration and incubation conditions, graphene oxide-chlorophyllin-Zn dispersion had the highest antibacterial activity, followed by graphene oxide-chlorophyllin and graphene oxide. Changes in the bacterial cell membrane were also investigated by field emission scanning electron microscopy. The incubation of bacteria with functionalized graphene oxide nanomaterials caused the loss of cellular integrity and consequently, cell death. The results suggested that the surface chemistry and metal toxicity played a major role in making graphene oxide-chlorophyllin-Zn a strong antibacterial agent. Antibacterial mechanisms of the functionalized graphene oxide nanomaterials are proposed.