TRI6 and TRI10 play different roles in the regulation of deoxynivalenol (DON) production by cAMP signalling in Fusarium graminearum
TRI6 and TRI10 play different roles in the regulation of deoxynivalenol (DON) production by cAMP signalling in Fusarium graminearum
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TRI6 和 TRI10 在禾谷镰刀菌中通过 cAMP 信号传导调节脱氧雪腐镰刀菌烯醇 (DON) 产生中发挥不同作用
DOI：
10.1111/1462-2920.13279
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发表时间：
2016-11-01
影响因子：
5.1
通讯作者：
Xu, Jin-Rong
中科院分区：
文献类型：
--
作者：
Jiang, Cong;Zhang, Chengkang;Xu, Jin-Rong
禾谷镰刀菌（Fusarium graminearum）毒素脱氧雪腐镰刀菌烯醇（deoxynivalenol，DON）的生物合成受两个途径特异性转录因子Tri6和Tri10的调控，并受多种宿主和环境因子的影响。在这项研究中，我们发现环磷酸腺苷（cAMP）处理通过刺激TRI基因表达和DON相关的细胞分化诱导F。禾谷早熟禾有趣的是，外源cAMP对tri6突变体没有影响，但部分恢复了tri10突变体在DON合成方面的缺陷。虽然PDE1和PDE2在营养生长、分生孢子形成、有性生殖和植物侵染中具有重叠的功能，但PDE2的缺失激活了胞内PKA活性，增加了DON的产生。而tri6 pde2突变体不能产生DON，tri10 pde2双突变体产生的DON水平显著高于tri10突变体。在tri10和tri6突变体中，与DON产生相关的细胞分化受到外源性cAMP或PDE2缺失的刺激。这些数据表明，TRI6是通过cAMP信号调节DON生物合成所必需的，但升高的PKA活性可以部分绕过TRI10对TRI基因表达和DON产生的要求，Pde 2是F.DON生物合成的主要cAMP磷酸二酯酶。禾谷早熟禾
The biosynthesis of mycotoxin deoxynivalenol (DON) in Fusarium graminearum is regulated by two pathway-specific transcription factors Tri6 and Tri10 and affected by various host and environmental factors. In this study, we showed that cyclic adenosine monophosphate (cAMP) treatment induced DON production by stimulating TRI gene expression and DON-associated cellular differentiation in F. graminearum. Interestingly, exogenous cAMP had no effects on the tri6 mutant but partially recovered the defect of tri10 mutant in DON biosynthesis. Although the two cAMP phosphodiesterase genes PDE1 and PDE2 had overlapping functions in vegetative growth, conidiation, sexual reproduction and plant infection, deletion of PDE2 but not PDE1 activated intracellular PKA activities and increased DON production. Whereas the tri6 pde2 mutant failed to produce DON, the tri10 pde2 double mutant produced a significantly higher level of DON than the tri10 mutant. Cellular differentiation associated with DON production was stimulated by exogenous cAMP or deletion of PDE2 in both tri10 and tri6 mutants. These data indicate that TRI6 is essential for the regulation of DON biosynthesis by cAMP signalling but elevated PKA activities could partially bypass the requirement of TRI10 for TRI gene-expression and DON production, and Pde2 is the major cAMP phosphodiesterase to negatively regulate DON biosynthesis in F. graminearum.