Physicochemical studies of the carcinoembryonic antigens of the human digestive system.
Physicochemical studies of the carcinoembryonic antigens of the human digestive system.
复制标题
人类消化系统的癌胚抗原的物理化学研究。
DOI：
10.1084/jem.128.3.387
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发表时间：
1968-09-01
影响因子：
15.3
通讯作者：
Freedman, S O
中科院分区：
文献类型：
--
作者：
Krupey, J;Gold, P;Freedman, S O
描述了一种纯化人类消化系统癌胚抗原(CEA)的方法。在0.6M高氯酸中提取肿瘤组织，然后用纸块电泳法和Sephadex G-200柱层析法得到高纯度的CEA制剂，经免疫学和理化标准鉴定。对5种不同来源的消化系统肿瘤转移癌胚抗原制剂的性质和组成进行了研究。调查结果显示，这些样本之间高度一致。沉积系数在6.9~8s之间。每个样本都显示存在14种不同的氨基酸残基和6种不同的碳水化合物成分(其中4种可以用可供分析的材料数量来定量)。对一例原发性肝癌的CEA纯化制剂的研究结果，在某些方面与转移性肿瘤来源的CEA样本的结果不同。对于肝癌制剂中可能存在的非CEA成分，这些变异的含义进行了讨论。最重要的是观察到，测试的少数正常成人消化系统组织未能显示出类似CEA的成分的存在。这一发现似乎表明，在成人中，人类消化系统的癌胚抗原在性质上是肿瘤特异性的，在可比的正常组织中无法检测到。
A procedure has been described for the purification of the carcinoembryonic antigens (CEA) of the human digestive system. Tumor tissue extraction in 0.6 M perchloric acid followed by paper block electrophoresis and column chromatography on Sephadex G-200 resulted in highly purified CEA preparations as determined by both immunological and physicochemical criteria. The properties and composition of five different purified CEA preparations derived from digestive system cancer metastases were examined. The findings demonstrated a high degree of uniformity amongst these samples. Sedimentation coefficients ranged from 6.9 to 8S. Each sample showed the presence of 14 different amino acid residues and six different carbohydrate constituents (four of which could be quantitated with the amount of material available for analyis). Studies of a purified CEA preparation from a primary hepatoma yielded results which, in some respects, differed from those obtained with the CEA samples of metastatic tumor origin. The implications of these variations were discussed with regard to the probable presence of non-CEA components in the hepatoma preparation. Of primary importance was the observation that the few normal adult digestive system tissues tested failed to show the presence of constituents similar to the CEA. This finding would seem to indicate that, in the adult, the carcinoembryonic antigens of the human digestive system are qualitatively tumor-specific and are not dectectable in comparable normal tissues.