Community-acquired MRSA and pig-farming.
Community-acquired MRSA and pig-farming.
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DOI：
10.1186/1476-0711-5-26
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发表时间：
2006-11-10
影响因子：
5.7
通讯作者：
de Neeling, Albert J
中科院分区：
文献类型：
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作者：
Huijsdens, Xander W;van Dijke, Beatrix J;de Neeling, Albert J
背景技术背景：CA-MRSA在没有MRSA携带风险因素的人群中的零星病例正在增加。病例介绍：我们报告了一个猪农，他的同事和他的猪的家庭成员之间的MRSA集群。最初，一位年轻的母亲被发现患有MRSA引起的乳腺炎。六个月后，她的小女儿因肺炎球菌性耳炎住院。在医院住了五天后，婴儿被发现是MRSA阳性。在这一点上，决定寻找一个可能的来源，如其他家庭成员和家养动物，包括农场的猪，因为这些被报告为MRSA的可能来源早些时候。从家庭成员和同事的喉咙和鼻孔中取出拭子。兽医从10头猪的鼻孔、喉咙和会阴获得拭子。按照国家方案培养拭子，以检测MRSA，包括使用增菌肉汤。通过PFGE、SPA分型、MLST分析、SSCmec、AGR分型以及PVL、LukM和TSST毒素基因的检测对动物和人菌株进行表征。三名家庭成员，三名同事和10头猪中的8头为MRSA阳性。除了最初的病例（母亲）之外，所有人都是单独的殖民地，没有临床感染的迹象。经SmaI酶切后，PFGE均未显示任何条带。所有菌株均为spa型t108和ST 398。该报告清楚地显示了荷兰人与猪之间的克隆传播和传播。2. MLST序列类型398可能是猪MRSA的国际重要性，因为这种类型被证明存在于流行病学无关的法国猪和养猪户。3.需要进行研究，以评估这是一个当地问题还是MRSA的新来源，这使得荷兰迄今为止成功的搜索和销毁政策处于危险之中。
BACKGROUND: Sporadic cases of CA-MRSA in persons without risk-factors for MRSA carriage are increasing.CASE PRESENTATION: We report a MRSA cluster among family members of a pig-farmer, his co-workers and his pigs. Initially a young mother was seen with mastitis due to MRSA. Six months later her baby daughter was admitted to the hospital with pneumococcal otitis. After staying five days in hospital, the baby was found to be MRSA positive. At that point it was decided to look for a possible source, such as other family members and house-hold animals, including pigs on the farm, since those were reported as a possible source of MRSA earlier. Swabs were taken from the throat and nares of family members and co-workers. A veterinarian obtained swabs from the nares, throat and perineum of 10 pigs. Swabs were cultured following a national protocol to detect MRSA that included the use of an enrichment broth. Animal and human strains were characterized by PFGE, spa-typing, MLST analysis, SSCmec, AGR typing, and the detection for PVL, LukM, and TSST toxin genes. Three family members, three co-workers, and 8 of the 10 pigs were MRSA positive. With the exception of the initial case (the mother) all persons were solely colonized, with no signs of clinical infections. After digestion with SmaI, none of the strains showed any bands using PFGE. All isolates belonged to spa type t108 and ST398.CONCLUSION: 1. This report clearly shows clonal spread and transmission between humans and pigs in the Netherlands. 2. MLST sequence type 398 might be of international importance as pig-MRSA, since this type was shown earlier to be present in epidemiologically unrelated French pigs and pig-farmers. 3. Research is needed to evaluate whether this is a local problem or a new source of MRSA, that puts the until now successful Search and Destroy policy of the Netherlands at risk.