Cloning and characterization of a mouse spergen-1 localized in sperm mitochondria.
Cloning and characterization of a mouse spergen-1 localized in sperm mitochondria.
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DOI：
10.1111/j.1365-2605.2004.00466.x
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发表时间：
2004-06
影响因子：
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通讯作者：
Y. Matsuoka;N. Iguchi;K. Kitamura;Hiromi Nishimura;Hiroyuki Manabe;Y. Miyagawa;M. Koga;K. Matsumiya;A. Okuyama;Hiromitsu Tanaka;Y. Nishimune
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文献类型：
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作者：
Y. Matsuoka;N. Iguchi;K. Kitamura;Hiromi Nishimura;Hiroyuki Manabe;Y. Miyagawa;M. Koga;K. Matsumiya;A. Okuyama;Hiromitsu Tanaka;Y. Nishimune
精子的发育是一个复杂的过程，包括鞭毛、细胞核和线粒体的特殊形态形成。虽然这些事件的详细形态学观察，分子机制仍有待充分阐明。我们在这里报告的分子克隆和表征的小鼠精子特异性线粒体蛋白，从一个单倍体生殖细胞特异性消减cDNA文库的小鼠睾丸。分离的cDNA是c. 0.7 kb，包含465 bp的ORF，编码小鼠精子原-1，一种由154个预测氨基酸组成的精子线粒体蛋白。针对小鼠Spergen-1产生的抗体鉴定了睾丸特异性c。Western blot分析显示18 Mr × 10（3）条带。该蛋白定位于小鼠精子的线粒体。小鼠和人类基因组序列的比较显示，含有CAAT盒和NF-κ B结合序列的55 bp的5 '上游区域是保守的，并且可能对小鼠精子原-1在单倍体生殖细胞中的特异性表达是重要的。
Development of spermatozoa is a complex process involving specific morphological formation of flagella, nucleus and mitochondria. Although detailed morphological observations of these events are available, the molecular mechanisms remain to be fully elucidated. We report here the molecular cloning and characterization of mouse spergen-1 encoding a sperm specific mitochondrial protein, from a haploid germ cell-specific subtracted cDNA library of mouse testis. Isolated cDNA is c. 0.7 kb and contains a 465 bp ORF that encodes mouse spergen-1, a sperm mitochondrial protein consisting of 154 predicted amino acids. Antibodies raised against mouse Spergen-1 identified a testis-specific c. 18 Mr x 10(3) band in Western blot analysis. The protein was localized to the mitochondria of mouse sperm. Comparison of the mouse and human genomic sequences showed that 55 bps of the 5'-upstream region containing a CAAT box and binding sequence for NF-kappa B is conserved and could be important for specific expression of mouse spergen-1 in haploid germ cells.