Efficacy of a B virus gD DNA vaccine for induction of humoral and cellular immune responses in Japanese macaques.
Efficacy of a B virus gD DNA vaccine for induction of humoral and cellular immune responses in Japanese macaques.
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B 病毒 gD DNA 疫苗诱导日本猕猴体液和细胞免疫反应的功效。
DOI：
10.1016/s0264-410x(02)00175-5
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发表时间：
2002
期刊：
影响因子：
5.5
通讯作者：
Eberle,Richard
中科院分区：
文献类型：
--
作者：
Hirano,Makoto;Nakamura,Shin;Mitsunaga,Fusako;Okada,Maki;Shimizu,Keiko;Ueda,Masahiro;Bennett,Alice;Eberle,Richard
人们希望防止 B 病毒 (BV) 在猕猴群体中传播，因为 BV 传播给人类会导致致命的脑脊髓炎。对猴子进行疫苗接种是限制 BV 在猕猴群体内传播的方法之一。用于猕猴的 BV DNA 疫苗的出现将消除感染传染性 BV 的风险。为此，我们构建了表达 BV 糖蛋白 D (gD) 的质粒。通过以每只 500μg 的剂量进行四次皮内注射，在成年日本猕猴中评估了该构建体作为 DNA 疫苗的免疫原性。使用重组单纯疱疹病毒 1 型 (HSV1) gD（BV gD 的同源物）进行的酶联免疫吸附测定 (ELISA) 结果显示，每次加强注射后，所有接种疫苗的动物都会诱导显着水平的抗体。接种疫苗的猕猴血清的蛋白质印迹证实了对真正的 BV gD 的特异性识别。免疫血清也被证明含有针对传染性 BV 的中和活性。使用重组 HSV1 gD 作为刺激抗原，在接种疫苗的猕猴中也观察到较弱的淋巴增殖反应，并且对一个个体的流式细胞术分析显示存在 HSV1 gD 反应性效应 T 细胞。因此，BV gD DNA 疫苗被证明可以在识别 BV gD 的猕猴中诱导体液和细胞免疫反应。
It is desirable to prevent dissemination of B virus (BV) in macaque colonies because transmission of BV to humans causes deadly encephalomyelitis. Vaccination of monkeys is one method that could confine spread of BV within macaque colonies. Availability of a BV DNA vaccine for use in macaques would eliminate the risk of working with infectious BV. Toward this end, we constructed a plasmid expressing the BV glycoprotein D (gD). Immunogenicity of this construct as a DNA vaccine was assessed in adult Japanese macaques by four intracutaneous injections at a dose of 500μg per head. Results of enzyme-linked immunosorbent assay (ELISA) using a recombinant herpes simplex virus type 1 (HSV1) gD, a homologue of BV gD, showed that significant levels of antibody was induced in all vaccinated animals following each booster injection. Western blot of sera from vaccinated macaques confirmed the specific recognition of authentic BV gD. Immune sera were also demonstrated to contain neutralizing activity against infectious BV. Weak lymphoproliferative responses were also observed in vaccinated macaques using recombinant HSV1 gD as a stimulating antigen and flow cytometry analysis of one individual revealed the presence of HSV1 gD-responsive effector T cells. Thus, the BV gD DNA vaccine was demonstrated to induce both humoral and cellular immune responses in macaques which recognized BV gD.