Dietary Corn Bran Fermented by Bacillus subtilis MA139 Decreased Gut Cellulolytic Bacteria and Microbiota Diversity in Finishing Pigs.
Dietary Corn Bran Fermented by Bacillus subtilis MA139 Decreased Gut Cellulolytic Bacteria and Microbiota Diversity in Finishing Pigs.
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枯草芽孢杆菌 MA139 发酵的日粮玉米麸减少育肥猪肠道纤维素分解细菌和微生物群多样性
DOI：
10.3389/fcimb.2017.00526
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发表时间：
2017
影响因子：
5.7
通讯作者：
Ma X
中科院分区：
文献类型：
--
作者：
Liu P;Zhao J;Guo P;Lu W;Geng Z;Levesque CL;Johnston LJ;Wang C;Liu L;Zhang J;Ma N;Qiao S;Ma X
枯草芽孢杆菌MA139对饲料进行固态发酵，可以降低体外不溶性膳食纤维含量，改善猪的生长性能。本研究旨在研究枯草杆菌发酵玉米皮对肥育猪生长性能和肠道微生物区系组成的影响。在21d的饲养试验中，将60头肥育猪随机分为对照组(CON)日粮、10%CB日粮和10%发酵CB(FCB)日粮。测定其生长性能和养分消化率。粪便标本通过16S rRNA基因扩增序列测定细菌群落多样性。饲粮CB和FCB对肥育猪的生长性能没有影响。CB和FCB处理组的有机物消化率均低于CON组(P<；0.05)。FCB处理的CHO 1细菌群落α多样性低于CON处理(P<；0.0 5)。分离到属于纤维分解菌的纤维杆菌门，CB组的相对丰度在CON和FCB处理之间没有差异。CB组乳螺科NK4A136_群的丰度高于Con组和FCB组(P<；0.05)，而FCB组的Norank_f_Prevoteaceae种群数量高于Con组和CB组(P<；0.05)。总之，饲料中的FCB降低了细菌群落的丰度，特别是与纤维素降解相关的细菌数量。
Solid-state fermentation of feedstuffs by Bacillus subtilis MA139 can reduce insoluble dietary fiber content in vitro and improve growth performance in pigs. This study was conducted to investigate the effects of dietary corn bran (CB) fermented by B. subtilis on growth performance and gut microbiota composition in finishing pigs. A total of 60 finishing pigs were allocated to 3 dietary treatments consisting of a control (CON) diet, a 10% CB diet, and a 10% fermented CB (FCB) diet in a 21 d feeding trial. Growth performance and nutrient digestibility were evaluated. Fecal samples were determined for bacterial community diversity by 16S rRNA gene amplicon sequencing. The dietary CB and FCB did not affect growth performance of finishing pigs. The digestibility of organic matter was decreased in both CB and FCB treatments compared with CON group (P < 0.05). The α-diversity for bacterial community analysis of Chao 1 in FCB treatment was lower than CON treatment (P < 0.05). The Fibrobacteres phylum belongs to cellulolytic bacteria was isolated, and their relative abundance in CB group showed no difference between CON and FCB treatments. The abundance of Lachnospiraceae_NK4A136_group in CB treatment was higher than CON and FCB groups (P < 0.05), whereas the population of norank_f_Prevotellaceae was higher in FCB group compared to CON and CB groups (P < 0.05). In conclusion, dietary FCB decreased the abundance of bacterial communities, particularly the population of bacteria related to cellulolytic degradation.