Detection of monoclonal antibody-defined colorectal carcinoma antigen by solid-phase binding inhibition radioimmunoassay.
Detection of monoclonal antibody-defined colorectal carcinoma antigen by solid-phase binding inhibition radioimmunoassay.
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通过固相结合抑制放射免疫分析检测单克隆抗体定义的结直肠癌抗原。
DOI：
10.1089/hyb.1.1981.1.37
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Koprowski,H
中科院分区：
文献类型：
--
作者：
Chang,TH;Steplewski,Z;Sears,HF;Koprowski,H
我们建立了固相结合抑制放射免疫分析法，用于检测人大肠癌细胞系组织培养上清和大肠癌患者血清及尿液中大肠癌特异性抗原。在该测定中使用[3 H]葡糖胺标记的细胞膜糖脂抗原和结直肠癌特异性单克隆抗体，我们已经能够检测从细胞膜释放到组织培养上清液中的几种人结直肠癌膜特异性抗原，以及由抗体1116-NS-19-9和1116-NS-52 a检测的抗原，其仅在癌症患者的血清和尿中发现。
We have established a solid-phase binding inhibition radioimmunoassay for the detection of colorectal carcinoma-specific antigens in tissue culture supernatants of human colorectal carcinoma cell lines and in serum and urine of colorectal carcinoma patients. Using the [3H]glucosamine-labeled cell membrane glycolipid antigen and colorectal carcinoma-specific monoclonal antibodies in this assay, we have been able to detect several human colorectal carcinoma membrane-specific antigens that are released from the cell membrane into tissue culture supernatants, and an antigen detected by antibodies 1116-NS-19-9 and 1116-NS-52a that is found only in the serum and urine of cancer patients.