Differential localization of lipid phosphate phosphatases 1 and 3 to cell surface subdomains in polarized MDCK cells
Differential localization of lipid phosphate phosphatases 1 and 3 to cell surface subdomains in polarized MDCK cells
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DOI：
10.1016/s0014-5793(03)00931-1
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发表时间：
2003-09-25
期刊：
影响因子：
3.5
通讯作者：
Kanoh, H
中科院分区：
文献类型：
--
作者：
Jia, YJ;Kai, M;Kanoh, H
脂质磷酸磷酸酶（LPPs）是一种完整的膜蛋白，具有六个跨膜结构域，作为胞外脂质磷酸去磷酸化的外酶。利用稳定表达人LPP1和LPP3的极化MDCK细胞，我们发现LPP1只位于细胞顶表面，而LPP3主要分布在基底外侧亚区。我们在LPP1的n端发现了一个新的由F(2)DKTRL(7)组成的顶端分选信号。在LPP3的情况下，存在于第二细胞质部分的二酪氨酸基序被确定为基底外侧靶向信号。我们的研究表明，LPP1和LPP3具有不同的分类信号，导致它们分别定位于根尖和基底外侧亚域。(C) 2003 Elsevier B.V.出版，版权所有。
Lipid phosphate phosphatases (LPPs) are integral membrane proteins with six transmembrane domains that act as ecto-enzymes dephosphorylating a variety of extracellular lipid phosphates. Using polarized MDCK cells stably expressing human LPP1 and LPP3, we found that LPP1 was located exclusively at the apical surface whereas LPP3 was distributed mostly in the basolateral subdomain. We identified a novel apical sorting signal at the N-terminus of LPP1 composed of F(2)DKTRL(7). In the case of LPP3, a dityrosine motif present in the second cytoplasmic portion was identified as basolateral targeting signal. Our work shows that LPP1 and LPP3 are equipped with distinct sorting signals that cause them to differentially localize to the apical vs. the basolateral subdomain, respectively. (C) 2003 Published by Elsevier B.V. All rights reserved.