UP-REGULATION OF ADHESION MOLECULES IN THE BRONCHIAL MUCOSA OF SUBJECTS WITH CHRONIC OBSTRUCTIVE BRONCHITIS
UP-REGULATION OF ADHESION MOLECULES IN THE BRONCHIAL MUCOSA OF SUBJECTS WITH CHRONIC OBSTRUCTIVE BRONCHITIS
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DOI：
10.1164/ajrccm.149.3.7509705
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发表时间：
1994-03-01
影响因子：
24.7
通讯作者：
SAETTA, M
中科院分区：
文献类型：
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作者：
DISTEFANO, A;MAESTRELLI, P;SAETTA, M
为了确定慢性支气管炎患者的支气管粘膜中粘附分子和细胞因子是否上调，我们对 16 名慢性支气管炎患者、8 名无症状吸烟者和 7 名正常不吸烟受试者进行了支气管活检。通过免疫组织化学检查支气管活检，以确定血管和支气管上皮上E-选择素和细胞间粘附分子-1（ICAM-1）的表达，以及粘膜下层细胞上白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、中性粒细胞弹性蛋白酶和嗜酸性粒细胞阳离子蛋白（EG-2）的表达。与无症状吸烟者 (p < 0.05) 和正常受试者 (p < 0.01) 相比，慢性支气管炎患者 E-选择素阳性血管数量增加。三组受试者中 ICAM-1 阳性血管、中性粒细胞以及 IL-1 β、TNF-α 和 EG-2 阳性细胞的数量没有显着差异。当根据是否存在气道阻塞来划分支气管炎组时，E-选择素阳性血管数量的增加仅在气道阻塞的支气管炎中持续存在，并且基底上皮细胞上ICAM-1的表达也增加。我们得出的结论是，在气道阻塞的慢性支气管炎的支气管粘膜中，血管上的E-选择素和基底上皮细胞上的ICAM-1表达增加，表明这些粘附分子参与了该疾病的发病机制。
To determine whether adhesion molecules and cytokines are upregulated in the bronchial mucosa of chronic bronchitics, we obtained bronchial biopsies in 16 chronic bronchitics, in eight asymptomatic smokers, and in seven normal nonsmoking subjects. Bronchial biopsies were examined by immunohistochemistry to identify the expression of E-selectin and intercellular adhesion molecule-1 (ICAM-1) on vessels and on bronchial epithelium, and the expression of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), neutrophil elastase, and eosinophil cationic protein (EG-2) on cells in the submucosa. Chronic bronchitics had an increased number of E-selectin-positive vessels when compared with both asymptomatic smokers (p < 0.05) and normal subjects (p < 0.01). The numbers of ICAM-1-positive vessels, neutrophils, and IL-1 beta-, TNF-alpha-, and EG-2-positive cells were not significantly different in the three groups of subjects examined. When the bronchitic group was divided according to the presence or absence of airway obstruction, the increased number of E-selectin-positive vessels persisted only in bronchitics with airway obstruction, who also had an increased expression of ICAM-1 on basal epithelial cells. We conclude that in the bronchial mucosa of chronic bronchitics with airway obstruction, there is an increased expression of E-selectin on vessels and of ICAM-1 on basal epithelial cells, suggesting the involvement of these adhesion molecules in the pathogenesis of the disease.