Surface-enhanced Raman sensors: early history and the development of sensors for quantitative biowarfare agent and glucose detection
Surface-enhanced Raman sensors: early history and the development of sensors for quantitative biowarfare agent and glucose detection
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DOI：
10.1002/jrs.1376
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发表时间：
2005-06-01
影响因子：
2.5
通讯作者：
Van Duyne, RP
中科院分区：
文献类型：
--
作者：
Haynes, CL;Yonzon, CR;Van Duyne, RP
表面增强拉曼光谱（Sers）是一种灵敏、选择性检测低浓度分析物的强有力技术。本文讨论了Sers的早期历史，必须理解的概念，以优化Sers的强度和基于SERS的传感器的发展。为了达到最低检测限，表面纳米结构和激光激发波长之间的关系，以及分析物/表面结合化学，必须仔细优化。这项工作利用纳米粒子的光学性质的高度可调的性质，建立第一组优化条件。当局域表面等离子体共振（LSPR）的能量位于激发波长的能量和感兴趣的振动带的能量之间时，Sers增强因子EFSERS被优化。利用本工作中使用的窄LSPR，可以直接实现类似于10（8）的EFSERS。利用这些优化条件开发基于SERS的传感器，用于两个重要的目标分子：炭疽芽孢杆菌生物标志物和血清蛋白混合物中的葡萄糖。使用这些优化的薄膜在纳米球表面，一个便宜的，便携式拉曼光谱仪成功地用于检测感染剂量的枯草芽孢杆菌孢子只有5-s的数据收集。用于检测枯草芽孢杆菌孢子的生物标志物不可逆地结合Sers底物，而其他重要的生物分子，如葡萄糖，对裸露的银表面没有任何可测量的结合亲和力。为了克服这一困难，在暴露于分析物溶液之前，在Sers基底上自组装生物相容性分隔层。使用分区层的方法来集中附近的SERS活性基板的葡萄糖，生理葡萄糖浓度可以检测，即使在干扰血清蛋白的存在下。版权所有（c）2005年约翰威利父子有限公司。
Surface-enhanced Raman spectroscopy (SERS) is a powerful technique for the sensitive and selective detection of low-concentration analytes. This paper includes a discussion of the early history of SERS, the concepts that must be appreciated to optimize the intensity of SERS and the development of SERS-based sensors. In order to achieve the lowest limits of detection, both the relationship between surface nanostructure and laser excitation wavelength, as well as the analyte/surface binding chemistry, must be carefully optimized. This work exploits the highly tunable nature of nanoparticle optical properties to establish the first set of optimization conditions. The SERS enhancement factor, EFSERS, is optimized when the energy of the localized surface plasmon resonance (LSPR) lies between the energy of the excitation wavelength and the energy of the vibrational band of interest. With the narrow LSPRs used in this work, it is straightforward to achieve EFSERS similar to 10(8). These optimization conditions were exploited to develop SERS-based sensors for two important target molecules: a Bacillus anthracis biomarker and glucose in a serum protein mixture. Using these optimized film-over-nanosphere surfaces, an inexpensive, portable Raman spectrometer was used successfully to detect the infectious dose of Bacillus subtilis spores with only a 5-s data collection. The biomarker used to detect the Bacillus subtilis spores binds irreversibly to SERS substrates, whereas other important biomolecules, such as glucose, do not have any measurable binding affinity to a bare silver surface. To overcome this difficulty, a biocompatible partition layer was self-assembled on the SERS substrate before exposure to the analyte solution. Using the partition layer approach to concentrate glucose near the SERS-active substrate, physiological glucose concentrations can be detected even in the presence of interfering serum proteins. Copyright (c) 2005 John Wiley & Sons, Ltd.