The rovA mutant of Yersinia enterocolitica displays differential degrees of virulence depending on the route of infection
The rovA mutant of Yersinia enterocolitica displays differential degrees of virulence depending on the route of infection
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DOI：
10.1128/iai.71.6.3512-3520.2003
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发表时间：
2003-06-01
影响因子：
3.1
通讯作者：
Miller, VL
中科院分区：
文献类型：
--
作者：
Dube, PH;Handley, SA;Miller, VL
小肠结肠炎耶尔森氏菌是一种侵袭性肠道病原体，可引起严重的炎症性疾病。最近，我们确定并表征了毒力的全球调节器（rovA）。当小鼠经口感染rovA突变体时，它们通过50%致死剂量（LD 50）分析而减毒，并且具有改变的感染动力学。最重要的是，与感染野生型Y染色体的小鼠相比，口服感染rovA突变体的小鼠极大地减少了派尔集合淋巴结的炎症。小肠结肠炎然而，我们在此提供的数据表明，当腹膜内（i.p.）递送rovA突变细菌时，它们的毒性明显高于口服给药。rovA突变体的腹腔注射LD 50仅比野生型Y高10倍。在小肠结肠炎中，存在对rovA突变体的显著炎症反应，这在肝脏和脾脏中是明显的。总之，这些数据表明，RovA调节子可能需要的早期事件发生在派尔集合淋巴结的感染。此外，这些数据表明，RovA调节子可能是Y。小肠结肠炎全身性疾病和炎症反应，如果派尔集合淋巴结被绕过。
Yersinia enterocolitica is an invasive enteric pathogen that causes significant inflammatory disease. Recently, we identified and characterized a global regulator of virulence (rovA). When mice are infected orally with the rovA mutant they are attenuated by 50% lethal dose (LD50) analysis and have altered kinetics of infection. Most significantly, mice orally infected with the rovA mutant have greatly reduced inflammation in the Peyer's patches compared to those infected with wild-type Y. enterocolitica. However, we present data here indicating that when the rovA mutant bacteria are delivered intraperitoneally (i.p.), they are significantly more virulent than when delivered orally. The i.p. LD50 for the rovA mutant is only 10-fold higher than that of the wild-type Y. enterocolitica, and there are significant inflammatory responses to the rovA mutant that are evident in the liver and spleen. Altogether, these data suggest that the RovA regulon may be required for the early events of the infection that occur in the Peyer's patches. Furthermore, these data suggest that the RovA regulon may be dispensable for Y. enterocolitica systemic disease and inflammatory responses if the Peyer's patches are bypassed.