TACTICS, an interactive platform for customized high-content bioimaging analysis

TACTICS, an interactive platform for customized high-content bioimaging analysis
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TACTICS,用于定制高内涵生物成像分析的互动平台

DOI:
10.1093/bioinformatics/btt035
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发表时间:
2013
期刊:
影响因子:
5.8
通讯作者:
S. Russell
S. Russell
中科院分区:
生物学3区
文献类型:
--
作者:
R. Shimoni;K. Pham;Mohammed Yassin;M. Gu;S. Russell

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我们描述了一个名为TACTICS的模块化MATLAB®,用于延时图像分析,满足当前可用软件包通常不提供的几个要求:(i)通过将数据端点直接链接到原始位置来评估提取的成像信息的质量的能力,(ii)每个参数的大规模并行分析,用于对图像的子群体内的参数之间的可能关系进行类似流式细胞术的评估,(iii)用于跟踪的用户控制的选项,例如限制分析区域的界面,(iv)自动化过程的手动校正,以及(v)用于与原始图像链接的跟踪后分析的用户界面,包括基于荧光比率测量来查看细胞谱系和归一化偏振比的选项。
We describe a modular MATLAB® Toolbox named TACTICS for time-lapse image analysis that meets several requirements not generally offered by currently available software packages: (i) the ability to assess quality of extracted imaging information by directly linking data end points to the original position, (ii) massively parallel analysis of each parameter, for flow cytometry-like assessment of possible relationships between parameters within sub-populations of the images, (iii) options for user control of the tracking such as an interface to restrict the analysis region, (iv) manual correction of automated processes and (v) user interfaces for post-tracking analysis that is linked to the original images, including options to view cell pedigrees and normalized polarization ratios based on fluorescence ratiometric measurements.
DOI: 10.1109/tip.2010.2069690
发表时间: 2010-12-01
影响因子: 10.6
作者:
Li, Chunming;Xu, Chenyang;Fox, Martin D.
通讯作者: Fox, Martin D.