Whi3 mnemon association with endoplasmic reticulum membranes confines the memory of deceptive courtship to the yeast mother cell.
Whi3 mnemon association with endoplasmic reticulum membranes confines the memory of deceptive courtship to the yeast mother cell.
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DOI：
10.1016/j.cub.2022.01.002
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发表时间：
2022-03-14
期刊：
影响因子：
--
通讯作者：
Caudron F
中科院分区：
文献类型：
--
作者：
Lau Y;Oamen HP;Grogg M;Parfenova I;Saarikangas J;Hannay R;Nichols RA;Hilvert D;Barral Y;Caudron F
Prion-like proteins are involved in many aspects of cellular physiology, including cellular memory. In response to deceptive courtship, budding yeast escapes pheromone-induced cell-cycle arrest through the coalescence of the G1/S inhibitor Whi3 into a dominant, inactive super-assembly. Whi3 is a mnemon (Whi3mnem), a protein that conformational change maintains as a trait in the mother cell but is not inherited by the daughter cells. How the maintenance and asymmetric inheritance of Whi3mnem are achieved is unknown. Here, we report that Whi3mnem is closely associated with endoplasmic reticulum (ER) membranes and is retained in the mother cell by the lateral diffusion barriers present at the bud neck. Strikingly, barrier defects made Whi3mnem propagate in a mitotically stable, prion-like manner. The amyloid-forming glutamine-rich domain of Whi3 was required for both mnemon and prion-like behaviors. Thus, we propose that Whi3mnem is in a self-templating state, lending temporal maintenance of memory, whereas its association with the compartmentalized membranes of the ER prevents infectious propagation to the daughter cells. These results suggest that confined self-templating super-assembly is a powerful mechanism for the long-term encoding of information in a spatially defined manner. Yeast courtship may provide insights on how individual synapses become potentiated in neuronal memory. Whi3 mnemon associates with endoplasmic reticulum membranes Diffusion barriers restricts Whi3 mnemon inheritance to the mother cell Whi3 prion-like domain can assemble in self-templating fibrils in vitro Whi3 can adopt a prion-like behavior in diffusion barrier-defective cells Lau et al. identify that the self-templating yeast mnemon Whi3 is retained in the mother cell during cell division after the memorization of a deceptive mating attempt through an association with compartmentalized endoplasmic reticulum membranes. This confinement is required to prevent the appearance of a mitotically stable prion-like behavior of Whi3.
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