THE PURIFICATION, STRUCTURE AND SYNTHESIS OF PROTICINE-3
THE PURIFICATION, STRUCTURE AND SYNTHESIS OF PROTICINE-3
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DOI：
10.1099/00222615-16-3-323
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发表时间：
1983-01-01
影响因子：
3
通讯作者：
SENIOR, BW
中科院分区：
文献类型：
--
作者：
SENIOR, BW
The ability of Proteus mirabilis to produce the bacteriocin, proticine 3, is found almost exclusively in strains that cause severe infections of the upper urinary tract. Proticine 3 was purified from lysates of mitomycin C-induced cultures. Biological activity was associated with structures resembling bacteriophage tails which, when first produced, were in the form of nails with one pointed end and a base plate with appendages at the other end. This form was unstable and changed to a rocket form in which the outer sheath contracted and thickened to reveal a protruding, hollow core that often became detached from the sheath. Purified proticine 3 comprises 2 major and 9 minor proteins. Fluorography showed that during production of the proticine, a 58,000 MW protein was synthesized late in the induction process and became the most intensely labeled protein in the culture. Synthesis of this protein coincided with the appearance and increase in titer of biologically-active proticine within the cell and with the appearance of nail forms. The protein is believed to be shed when an active nail is converted to an inactive rocket and to be either the component of proticine 3 associated with its lethal activity, or the protein required for the correct assembly of the constituent components into a biological-active particle. The role of proticine 3 as a virulence factor is discussed.
