Neochloris oleabundans UTEX #1185: a suitable renewable lipid source for biofuel production
Neochloris oleabundans UTEX #1185: a suitable renewable lipid source for biofuel production
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DOI：
10.1007/s10295-009-0559-2
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发表时间：
2009-06-01
影响因子：
3.4
通讯作者：
Reis, Alberto
中科院分区：
文献类型：
--
作者：
Gouveia, Luisa;Marques, Ana Evangelista;Reis, Alberto
能源危机、全球变暖和气候变化要求在利用非常规原料制造高质量运输燃料方面取得技术和商业进步。微藻是最有希望的生物燃料来源之一，因为它每单位面积的产量很高，而且不会取代粮食作物。油丰新绿藻（Neo）微藻是单细胞油脂（SCO）的重要微生物来源。通过光密度（540 nm）、干重测定和流式细胞术（FC）对不同的实验生长和脂质产生条件进行评估和比较。在充氮和补充CO(2)条件下（N+/30A℃/CO(2)），在30℃条件下获得最佳的Neo平均生物量生产力，平均翻倍时间为1.4 d。在26A°C （N+/26A°C）和30A°C （N+/30A°C）条件下，不添加CO(2)的富氮培养的产量分别达到第二和第三高，分别增加了1.7和2.2天。用尼罗红（NR）流式细胞术监测微生物脂质生成，尼罗红是一种亲脂荧光染料，具有近实时（在线）原位筛选的几个有利特性。结果表明，在不补充CO(2)的氮饥饿（N-/30A℃）条件下，6 d时脂肪含量最高（56%），其次是N-/30A℃/CO(2)和N-/26A℃条件下，5 d和6 d时脂肪含量分别为52%。Neo脂质中充足的脂肪酸和碘值增强了这种微藻作为SCO的良好来源，特别是用作生物柴油。
Energy crises, global warming, and climatic changes call for technological and commercial advances in manufacturing high-quality transportation fuels from unconventional feedstocks. Microalgae is one of the most promising sources of biofuels due to the high yields attained per unit area and because it does not displace food crops. Neochloris oleabundans (Neo) microalga is an important promising microbial source of single-cell oil (SCO). Different experimental growth and lipid production conditions were evaluated and compared by using optical density (540 nm), dry-weight determination, and flow cytometry (FC). Best Neo average biomass productivity was obtained at 30A degrees C under conditions of nitrogen-sufficiency and CO(2) supplementation (N+/30A degrees C/CO(2)), with an average doubling time of 1.4 days. The second and third highest productivities occurred with N-sufficient cultures without CO(2) supplementation at 26A degrees C (N+/26A degrees C) and at 30A degrees C (N+/30A degrees C), with doubling times of 1.7 and 2.2 days, respectively. Microbial lipid production was monitored by flow cytometry using Nile red (NR), a lipophilic fluorochrome that possesses several advantageous characteristics for in situ screening near real time (at line). Results showed maximum lipid content (56%) after 6 days of nitrogen depletion under nitrogen starvation without CO(2) supplementation (N-/30A degrees C), followed by N-/30A degrees C/CO(2) and N-/26A degrees C conditions with 52% lipid content, after 5 and 6 days of N starvation, respectively. The adequate fatty acid profile and iodine value of Neo lipids reinforced this microalga as a good source of SCO, in particular for use as biodiesel.