Virus RNA persists within the retina in coronavirus-induced retinopathy
Virus RNA persists within the retina in coronavirus-induced retinopathy
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DOI：
10.1006/viro.1996.0442
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发表时间：
1996-08-15
期刊：
影响因子：
3.7
通讯作者：
Hooks, JJ
中科院分区：
文献类型：
--
作者：
Komurasaki, Y;Nagineni, CN;Hooks, JJ
鼠冠状病毒、小鼠肝炎病毒 (MHV)、JHM 株可诱导成年 BALB/c 小鼠出现双相性视网膜疾病。在早期阶段，即第 1 天到第 7 天，注意到视网膜血管炎，这与病毒蛋白和传染性病毒的存在有关。在晚期，即第 10 天到第 140 天，视网膜变性与病毒蛋白、传染性病毒和炎症细胞的缺失有关。本研究的目的是确定在疾病的视网膜退行性阶段病毒 RNA 是否持续存在于视网膜内。 BALB/c小鼠通过玻璃体内途径接种10(4.0)TCID50/5μl病毒。通过与病毒 cDNA 探针进行原位杂交来检测病毒 RNA 的存在，并通过免疫细胞化学染色来鉴定病毒蛋白。在感染的急性期，在视网膜、RPE、睫状体上皮和虹膜上皮中发现了病毒RNA。在感染后期，病毒 RNA 几乎只在视网膜和 RPE 中发现，而不是在眼前段。在视网膜内，神经节细胞层、视网膜内层、视网膜外层和视网膜色素上皮细胞中检测到病毒RNA。免疫细胞化学染色仅在第 1 天至第 8 天期间在视网膜内鉴定出病毒蛋白。这种眼部疾病还与持续的全身感染有关。前 8 天，病毒 RNA 和病毒蛋白均在肝脏内被鉴定出来。然而，从第 8 天到第 60 天，在肝脏中仅检测到病毒 RNA。这些研究表明，MHV 建立了急性感染（第 1-8 天），其中鉴定出传染性病毒和病毒蛋白。随后，视网膜和肝脏内出现持续感染，通过原位杂交仅检测到病毒 RNA。 (C) 1996 学术出版社
The murine coronavirus, mouse hepatitis virus (MHV), JHM strain, induces a biphasic retinal disease in adult BALB/c mice. In the early phase, Day 1 to Day 7, a retinal vasculitis is noted which is associated with the presence of viral proteins and infectious virus. In the late phase, Day 10 to Day 140, a retinal degeneration is associated with the absence of viral proteins, infectious virus, and inflammatory cells. The purpose of this study was to determine if viral RNA persists within the retina during the retinal degenerative phase of the disease. BALB/c mice were inoculated by the intravitreal route with 10(4.0) TCID50/5 mu l of virus. The presence of viral RNA was detected by in situ hybridization with a viral cDNA probe and viral proteins were identified by immunocytochemical staining. During the acute phase of the infection, viral RNA was found in the retina, RPE, ciliary body epithelium, and the iris epithelium. During the late phase of the infection, viral RNA was almost exclusively found within the retina and RPE and not in the anterior segment of the eye. Within the retina, viral RNA was detected in the ganglion cell layer, the inner retina, the outer retina, and the RPE cell. Immunocytochemical staining identified viral protein within the retina only from Day 1 to Day 8, This ocular disease was also associated with a persistent systemic infection. Both viral RNA and viral proteins were identified within the liver during the first 8 days. However, only viral RNA was detected in the liver from Day 8 to Day 60. These studies demonstrate that MHV established an acute infection (Day 1-8) where infectious virus and viral proteins were identified. This was followed by a persistent infection within the retina and liver where only viral RNA were detected by in situ hybridization. (C) 1996 Academic Press, Inc.