Conformational Changes in the Solution Structure of the Dengue Virus 5′ End in the Presence and Absence of the 3′ Untranslated Region
Conformational Changes in the Solution Structure of the Dengue Virus 5′ End in the Presence and Absence of the 3′ Untranslated Region
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DOI：
10.1128/jvi.01362-08
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发表时间：
2009-01-15
影响因子：
5.4
通讯作者：
Harris, Eva
中科院分区：
文献类型：
--
作者：
Polacek, Charlotta;Foley, Jonathan E.;Harris, Eva
登革热病毒 (DENV) 是一种类似于 10.7 kb 正义 RNA 病毒，通过 5' 和 3' 末端区域序列之间的 RNA-RNA 相互作用进行环化。环化序列 (CS) 和上游 AUG 区域 (UAR) 之间的互补性已被证明对于病毒复制是必要的。在这里，我们展示了 2 型 DENV 5' 末端存在和不存在 3' 末端的溶液结构。我们证明5'和3'CS之间的杂交独立于UAR，而5'UAR-3'UAR杂交依赖于5'CS-3'CS相互作用。
Dengue virus (DENV) is an similar to 10.7-kb positive-sense RNA virus that circularizes via RNA-RNA interactions between sequences in the 5' and 3' terminal regions. Complementarity between the cyclization sequence (CS) and the upstream AUG region (UAR) has been shown to be necessary for viral replication. Here, we present the solution structure of the 5' end of DENV type 2 in the presence and absence of the 3' end. We demonstrate that hybridization between the 5' and 3' CSs is independent of the UAR while the 5' UAR-3' UAR hybridization is dependent upon the 5' CS-3' CS interaction.