Human umbilical cord mesenchymal stem cells protect against ischemic brain injury in mouse by regulating peripheral immunoinflammation
Human umbilical cord mesenchymal stem cells protect against ischemic brain injury in mouse by regulating peripheral immunoinflammation
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人脐带间充质干细胞通过调节外周免疫炎症预防小鼠缺血性脑损伤
DOI：
10.1016/j.brainres.2014.10.065
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发表时间：
2015-01-12
期刊：
影响因子：
2.9
通讯作者：
Xu, Yun
中科院分区：
文献类型：
--
作者：
Cheng, Qiantao;Zhang, Zhuo;Xu, Yun
目前对缺血性中风的治疗是有限的，干细胞移植作为一种治疗策略提供了巨大的潜力。本研究旨在确定人脐带源性间充质干细胞（hUC-MSCs）是否可以通过调节周围免疫炎症来改善大脑中动脉闭塞（MCAO）后的脑损伤。研究表明，在脑卒中后30分钟内通过尾静脉注射hUC-MSCs，在mcao后72 h至1周内，神经功能缺损得到改善，脑水肿、梗死体积显著减少；hUC-MSCs可降低脑卒中后外周血及缺血半球炎症因子IL-1、tnf - α、IL-23、IL-17、IL-10水平；hUC-MSCs在mcao后24 h显著降低外周免疫系统Th17细胞水平，72 h显著升高Tregs水平；hUC-MSCs可提高血清tgf - β水平。总之，我们的研究结果表明，hUC-MSCs通过tgf - β调节外周免疫对MCAO小鼠具有神经保护作用，hUC-MSCs可能作为缺血性卒中的潜在治疗方法。(C) 2014 Elsevier B.V.版权所有
Current treatments for ischemic stroke are limited, stem cell transplantation offers great potential as a therapeutic strategy. The present study was undertaken to determine whether human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) could improve brain injury after middle cerebral artery occlusion (MCAO) through modulating peripheral immunoinflammation. The study showed that neurological deficit was ameliorated and brain edema, infarct volume was significantly decreased from 72 h to 1 week post-MCAO with hUC-MSCs treatment via tail vein injection within 30 mins after stroke; hUC-MSCs attenuated the levels of inflammatory factors including IL-1, TNF-alpha, IL-23, IL-17 and IL-10 in peripheral blood serum and ischemia hemisphere after stroke; hUC-MSCs significantly decreased the level of Th17 cells at 24 h and increased the level of Tregs at 72 h post-MCAO in peripheral immune system; the level of TGF-beta in blood serum was enhanced by hUC-MSCs. In conclusion, our findings suggested that hUC-MSCs had neuroprotection in MCAO mice by TGF-beta modulating peripheral immune and hUC-MSCs may be as a potential therapy for ischemic stroke. (C) 2014 Elsevier B.V. All rights reserved.